A PHP Error was encountered

Severity: Warning

Message: file_get_contents(https://...@gmail.com&api_key=61f08fa0b96a73de8c900d749fcb997acc09): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests

Filename: helpers/my_audit_helper.php

Line Number: 143

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 143
Function: file_get_contents

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 209
Function: simplexml_load_file_from_url

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3098
Function: getPubMedXML

File: /var/www/html/application/controllers/Detail.php
Line: 574
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 488
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

A PHP Error was encountered

Severity: Warning

Message: Attempt to read property "Count" on bool

Filename: helpers/my_audit_helper.php

Line Number: 3100

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3100
Function: _error_handler

File: /var/www/html/application/controllers/Detail.php
Line: 574
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 488
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

A Strain near Isogenic to the Sequenced FGSC7600 Strain for Producing Homozygous Multigene Mutants. | LitMetric

A Strain near Isogenic to the Sequenced FGSC7600 Strain for Producing Homozygous Multigene Mutants.

J Fungi (Basel)

Toxicology & Mycotoxin Research Unit, Agricultural Research Service, United States Department of Agriculture, US National Poultry Research Center, Athens, GA 30605, USA.

Published: August 2024

AI Article Synopsis

  • - The study focuses on creating a nearly identical (isogenic) fungal strain to the wild-type strain FGSC7600 by using sexual reproduction and specific breeding techniques to reduce genetic variability.
  • - The researchers conducted six backcross generations after an initial cross, mapping genetic recombination events and confirming inheritance patterns, which revealed some chromosomes remained intact while others had deletions.
  • - The resulting strain, TMRU10/35, shows about 93% genetic similarity to FGSC7600 and is available for further research through designated culture collections.

Article Abstract

Fungal genetic systems ideally combine molecular tools for genome manipulation and a sexual reproduction system to create an informative assortment of combinations of genomic modifications. When employing the sexual cycle to generate multi-mutants, the background genotype variations in the parents may result in progeny phenotypic variation obscuring the effects of combined mutations. Here, to mitigate this variation in , we generated a strain that was near isogenic to the sequenced wild-type strain, FGSC7600. This was accomplished by crossing FGSC7600 with the divergent wild-type strain FGSC7603 followed by six sequential backcrosses (e.g., six generations) of progeny to FGSC7600. We sequenced each generation and mapped recombination events. The parental cross involved twenty-six crossovers on nine of the eleven chromosomes. The dispensable chromosome 12, found in FGSC7603 but lacking in FGSC7600, was not present in the progeny post generation five. Inheritance of complete chromosomes without crossover was frequently observed. A deletion of approximately 140 kilobases, containing 54 predicted genes on chromosome 4, occurred in generation 4 and was retained in generation 5 indicating that these genes are dispensable for growth and both asexual and sexual reproduction. The final strain TMRU10/35 is about 93% identical to FGSC7600. TMRU10/35 is available from the Fungal Genetics Stock Center as FGSC27326 and from the ARS Culture Collection as NRRL64809.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11355202PMC
http://dx.doi.org/10.3390/jof10080592DOI Listing

Publication Analysis

Top Keywords

strain isogenic
8
isogenic sequenced
8
sexual reproduction
8
wild-type strain
8
strain
6
fgsc7600
6
sequenced fgsc7600
4
fgsc7600 strain
4
strain producing
4
producing homozygous
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!