AI Article Synopsis
- - The Mediterranean mollusc has experienced population declines due to a significant mortality event linked to a parasite, but some localized populations have shown resilience possibly because of their specific environmental conditions.
- - Researchers developed a molecular multiplex PCR method to accurately identify species and detect hybrids, using species-specific primers targeting the Internal Transcribed Spacer (ITS) regions of selected bivalves.
- - The study analyzed samples from various Mediterranean areas, confirming species differentiation through DNA amplification and sequencing, thereby demonstrating the importance of molecular tools in conserving biodiversity and managing hybridization.
Article Abstract
The , a Mediterranean mollusc, has suffered population declines due to a massive mortality event associated with various factors including the parasite . Some populations show resilience, possibly due to local environmental conditions. In this study, a molecular multiplex PCR method was developed using species-specific primers targeting Internal Transcribed Spacer (ITS) regions of and , allowing accurate species identification and hybrid detection. Samples from Mediterranean areas were analysed, including putative hybrids and individuals from five other bivalve species. DNA was isolated, ITS regions were amplified and sequenced, and phylogenetic analyses confirmed species differentiation and primer specificity. The multiplex-PCR successfully identified , , and their hybrids based on distinct amplicon patterns. This study highlights the value of molecular tools in species conservation, especially for monitoring and managing hybridization, supporting effective biodiversity conservation strategies.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11347391 | PMC |
| http://dx.doi.org/10.1002/ece3.70227 | DOI Listing |
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