AI Article Synopsis
- The study aimed to identify factors that influence plasma clot retraction and improve the experimental methods for analyzing this process, focusing on platelet function regulation and drug effects.
- Researchers experimented with varying concentrations of thrombin, calcium ions, and platelets to refine a detection system for plasma clot retraction, ensuring its reliability by testing various signaling pathway inhibitors.
- Results indicated that using platelet rich plasma with specific thrombin and calcium concentrations led to significant plasma clot retraction, and confirmed that certain inhibitors could notably affect this retraction process.
Article Abstract
Objective: To explore the key factors affecting plasma clot retraction and optimize the experimental method of plasma clot retraction, in order to study the regulation of platelet function and evaluate the modulatory effects of drugs on plasma clot retraction.
Methods: The effects of different concentrations of thrombin, Ca2 and platelets on plasma clot retraction were studied, and the detection system of plasma clot retraction was optimized. The availability of the detection system was then validated by analyzing the regulatory effects of multiple signaling pathway inhibitors on plasma clot retraction.
Results: Through the optimization study of multiple factors, platelet rich plasma (PRP) containing 0.5 mmol/L Ca2 and 40×10/L platelets was treated with 0.2 U/ml thrombin to perform plasma clot retraction analysis. After treatment with thrombin for 15 min, plasma clot retracted significantly. After treatment with thrombin for 30 min, the percentage of plasma clot retraction was more than 50%. The regulatory effects of multiple signaling pathway inhibitors on plasma clot retraction were studied in this detection system. PKC inhibitor Go 6983 exhibited a significant inhibitory effect on plasma clot retraction, while PI3K inhibitor Ly294002 and p38 MAPK inhibitor SB203580 slightly suppressed plasma clot retraction.
Conclusion: PRP containing 0.5 mmol/L Ca2 and 40×10/L platelets can be induced with 0.2 U/ml thrombin to conduct plasma clot retraction analysis, which can be used to study the regulation of platelet function and evaluate the modulatory effects of drugs on plasma clot retraction.
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| http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2024.04.045 | DOI Listing |
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