Short communications: Exploring temporal fluorescent changes in the composition of human semen stains.

J Chromatogr B Analyt Technol Biomed Life Sci

Amsterdam University Medical Centers (Amsterdam UMC), Location AMC, University of Amsterdam, Biomedical Engineering and Physics, Meibergdreef 9, Amsterdam, The Netherlands; Amsterdam Public Health Research Insitute (APH), Amsterdam, The Netherlands; Amsterdam University of Applied Science, Forensic Science, Tafelbergweg 51, Amsterdam, The Netherlands. Electronic address:

Published: October 2024

Semen traces are considered important pieces of evidence in forensic investigations, especially those involving sexsual offenses. Recently, our research group developed a fluorescence-based technique to accurately determine the age of semen traces. However, the specific compounds resonsible for the fluoresescent behaviour of ageing semens remain unknown. As such, in this exploratory study, the aim is to identify the components associated with the fluorescent behavior of ageing semen traces. In this investigation semen stains and various biofluorophores commonly found in body fluids were left to aged for 0, 2, 4, 7, 14 and 21 days. Subsequently, thin-layer chromatography (TLC) and ultra-performance liquid chromatography (UPLC) mass spectrometry were performed to identify the biofluorophores present in semen. Several contributors to the autofluorescence could be identified in semen stain, these include tryptophan, kynurenine, kynurenic acid, and norharman. The study sheds light on the.

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http://dx.doi.org/10.1016/j.jchromb.2024.124278DOI Listing

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