The budding yeast is a powerful model organism, partly because of the ease of genome alterations due to the combination of a fast generation time and many molecular genetic tools. Recent advances in CRISPR-based systems allow for the easier creation of alleles with internally inserted sequences within the coding regions of genes, such as the internal insertion of sequences that code for epitopes or fluorescent proteins. Here we briefly summarize some exisiting nomenclature standards and suggest nomenclature guidelines for internal insertion alleles which are informative, consistent, and computable.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11342080PMC
http://dx.doi.org/10.17912/micropub.biology.001258DOI Listing

Publication Analysis

Top Keywords

internal insertion
12
insertion alleles
8
naming internal
4
alleles created
4
created crispr
4
crispr budding
4
budding yeast
4
yeast powerful
4
powerful model
4
model organism
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!