Unlabelled: This study was conducted to propose a new strategy for preventing -mediated potato soft rot through the development of a carboxymethyl cellulose (CMC)-based antibacterial coating incorporated with green tea extract (GTE). GTE/CMC films resulted in increased water vapor permeability due to the incorporation of polar groups in GTE. In the antibacterial test against , the MBC value of GTE was 2 mg/mL. The time-kill assay demonstrated that GTE/CMC (2 × MBC) completely eradicated bacteria within 0.5 h (~6.4 log CFU/mL reduction). The potential of GTE/CMC to prevent potato soft rot was evaluated by monitoring the potato appearance, maceration area, and texture properties. The GTE/CMC-coated potatoes exhibited significantly reduced maceration area and remained firm for 3 days. Moreover, there was no change in the antimicrobial efficacy for 8 weeks. The developed GTE/CMC could be used as a biological coating system for postharvest storage and soft rot prevention.
Supplementary Information: The online version contains supplementary material available at 10.1007/s10068-024-01548-6.
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http://dx.doi.org/10.1007/s10068-024-01548-6 | DOI Listing |
Microorganisms
November 2024
A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.
is a bacterial phytopathogen that causes soft and black rot and actively spreads worldwide. Our study is the first development of immunoassays for detecting . We immunized rabbits and obtained serum with an extremely high titer (1:10).
View Article and Find Full Text PDFPathogens
December 2024
Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT 84602, USA.
Bacterial soft rot causes major crop losses annually and can be caused by several species from multiple genera. These bacteria have a broad host range and often infect produce through contact with soil. The main genera causing bacterial soft rot are and , both of which have widespread geographical distribution.
View Article and Find Full Text PDFInt J Mol Sci
December 2024
Department of Oral and Maxillofacial Plastic Surgery, Faculty of Medicine, Martin Luther University Halle-Wittenberg, 06120 Halle, Germany.
The regulator of the canonical Wnt pathway, leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5), is expressed in the stem cell compartment of several tissues and overexpressed in different human carcinomas. The isoform of the stem cell marker LGR5, named LGR5Δ5 and first described by our group, is associated with prognosis and metastasis in oral squamous cell carcinoma (OSCC) and soft tissue sarcoma (STS). In a proof-of-principle analysis, the function of LGR5Δ5 was investigated in HEK293T cells, a model cell line of the Wnt pathway, compared to full-length LGR5 (FL) expression.
View Article and Find Full Text PDFBiology (Basel)
December 2024
Key Laboratory City for Study and Utilization of Ethnic Medicinal Plant Resources of Western Guizhou Province, Liupanshui Normal University, Liupanshui 553004, China.
Pathogen-induced fruit decay is a significant threat to the kiwifruit industry, leading to considerable economic losses annually. The cell-wall-degrading enzymes (CWDEs) secreted by these pathogens are crucial for penetrating the cell wall and accessing nutrients. Among them, species are recognized as major causal agents of soft rot in kiwifruit, yet their pathogenic mechanisms are not well understood.
View Article and Find Full Text PDFPlant Dis
January 2025
University of Ghana College of Basic and Applied Sciences, Biotechnology Centre, Accra, Greater Accra, Ghana;
African eggplant (Solanum aethiopicum gilo group) is a nutritious vegetable widely commercialized in Ghana. In the 2021 planting season (May-July), collar rot symptoms were observed on African eggplant on a farm at Domeabra, Legon, and Okumaning in the Central (N5° 48' 11″, W1° 26' 48″), Greater Accra (N5° 39' 34″, W0° 11' 34″) and Eastern (N6° 8' 34″, W0° 55' 59″) regions of Ghana, respectively. Disease incidence was 8-15% in the different farms.
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