AI Article Synopsis
- Allergic contact dermatitis (ACD) from protective gloves can arise from rubber additives, and a newly identified allergen, 2-cyanoethyl dimethyldithiocarbamate (CEDMC), has been found in accelerator-free rubber gloves, causing ACD in some individuals.
- The study explored the effects of CEDMC, along with two other reference sensitizers, using a dendritic cell model to analyze cellular responses through various scientific methods, including transcriptomics and flow cytometry.
- Results showed that CEDMC and the reference chemicals were strong skin sensitizers, increasing certain immune markers and cytokine secretion, while also inducing apoptosis, which provides insights into the chemical mechanisms behind skin sensitization from rubber materials.
Article Abstract
Background: Allergic contact dermatitis (ACD) from protective gloves is often caused by rubber additives, such as accelerators. However, while accelerator-free rubber gloves are available, they still cause ACD in some individuals.
Objectives: A new allergen, 2-cyаnоethyl dimethyldithiocarbamate, (CEDMC), has recently been identified in accelerator-free gloves, and we here provide a first in vitro characterisation of CEDMC in a dendritic cell (DC)-like cell model along with three reference sensitizer rubber chemicals, consisting of tetraethylthiuram disulfide (TETD) and two xanthogenates.
Methods: Cellular responses after the exposure to the rubber chemicals were assessed using a transcriptomic approach, multiplex cytokine secretion profiling, and flow cytometry to determine DC model activation marker expression and apoptosis induction.
Results: CEDMC and all other sensitizers were classified as strong skin sensitizers with the transcriptomic approach. They all significantly increased IL-8 secretion and exposure to all except one increased CD86 DC activation marker expression. When tested, CEDMC induced apoptosis, however, delayed compared to TETD.
Conclusions: The in vitro data corroborate CEDMC, TETD, and investigated xanthogenates as skin sensitizers. Transcriptomic analyses further reveal unique cellular responses induced by CEDMC, which together with future study can contribute to better understanding of cellular mechanisms underlying the sensitising capacity of rubber chemicals.
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Source |
|---|---|
| http://dx.doi.org/10.1111/cod.14682 | DOI Listing |
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669566 | PMC |
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