AI Article Synopsis
- * Researchers established a new silkworm infection model to assess the virulence of Mycobacterium intracellulare subspecies within just 4 days.
- * The study found that the median lethal dose (LD) values indicated that M. intracellulare subsp. chimaera was less virulent than M. intracellulare subsp. intracellulare, confirming the model's effectiveness in comparing subspecies virulence.
Article Abstract
Non-tuberculous mycobacteria (NTM) cause skin infections, respiratory diseases, and disseminated infections. Mycobacterium avium and Mycobacterium intracellulare, which are slow grown Mycobacterium, are main agents of those NTM diseases. A silkworm infection model with Mycobacterium abscessus, a rapidly growing Mycobacterium species, was established to quantitatively evaluate its virulence within a short period. However, a silkworm infection model to quantitatively evaluate the virulence of M. intracellulare has not yet been developed. In this study, we determined the virulence of M. intracellulare subspecies within 4 days using a silkworm infection model. The subspecies of M. intracellulare strains used in this study were estimated by phylogenetic tree analysis using core gene data. The median lethal dose (LD) values, which are the dose of a pathogen required to kill half of the silkworms in a group, were determined 4 days after infection. The LD value of M. intracellulare subsp. chimaera DSM44623 was higher than that of M. intracellulare subsp. intracellulare ATCC13950. These results suggest that the virulence of M. intracellulare subspecies can be compared using a silkworm model within 4 days.
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| http://dx.doi.org/10.5582/ddt.2024.01043 | DOI Listing |
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