AI Article Synopsis
- * The study developed a MALDI-MS imaging method to visualize oxidized oligosaccharides produced by LPMOs in maize plant tissues, revealing their action at the cellular level.
- * The research highlights that LPMOs selectively target specific areas of the plant cell wall, influenced by the wall's composition and structure.
Article Abstract
Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that oxidatively cleave recalcitrant polysaccharides such as cellulose. Several studies have reported LPMO action in synergy with other carbohydrate-active enzymes (CAZymes) for the degradation of lignocellulosic biomass but direct LPMO action at the plant tissue level remains challenging to investigate. Here, we have developed a MALDI-MS imaging workflow to detect oxidised oligosaccharides released by a cellulose-active LPMO at cellular level on maize tissues. Using this workflow, we imaged LPMO action and gained insight into the spatial variation and relative abundance of oxidised and non-oxidised oligosaccharides. We reveal a targeted action of the LPMO related to the composition and organisation of plant cell walls.
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| http://dx.doi.org/10.1016/j.carbpol.2024.122465 | DOI Listing |
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