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Myocyte enhancer factor 2 upregulates expression of myostatin promoter in Yesso scallop, Patinopecten yessoensis. | LitMetric

Myocyte enhancer factor 2 upregulates expression of myostatin promoter in Yesso scallop, Patinopecten yessoensis.

Comp Biochem Physiol B Biochem Mol Biol

State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Laoshan Laboratory, Qingdao, Shandong 266237, China. Electronic address:

Published: January 2025

AI Article Synopsis

  • Myostatin (MSTN) is crucial for muscle development in animals, but its regulation in marine invertebrates, like bivalves, is not well understood.
  • The study involved cloning the MSTN promoter sequence from the Yesso scallop, discovering multiple transcription start sites and binding sites for transcription factors like MEF2.
  • The research showed that MEF2 significantly enhances MSTN transcriptional activity, which implies its role in regulating muscle growth in the Yesso scallop.

Article Abstract

Myostatin (MSTN) plays an important role in muscle development in animals, especially for mammals and fishes. However, little information has been reported on the regulation of MSTN in marine invertebrates, such as bivalves. In the present study, we cloned the MSTN promoter sequence of Yesso scallop Patinopecten yessoensis, identifying 4 transcription start sites, eleven TATA boxes and one E-box. Additionally, transcription factor binding sites, including myocyte enhancer factor 2 (MEF2) and POU homeodomain protein, were identified. The interaction between the MSTN promoter and MEF2 was analyzed to reveal the transcriptional activity of different fragment sizes of promoters through the dual-luciferase reporter assays. The highest transcriptional activity was found in recombinant plasmids with the most MEF2 binding sites, indicating that this transcription factor upregulates MSTN in Yesso scallop. This study provides new insight into the regulation of muscle growth and development in this species.

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Source
http://dx.doi.org/10.1016/j.cbpb.2024.111024DOI Listing

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