AI Article Synopsis
Article Abstract
Avian primordial germ cells (PGCs) are important culture cells for the production of transgenic chickens and preservation of the genetic resources of endangered species; however, culturing these cells in vitro proves challenging. Although the proliferation of chicken PGCs is dependent on insulin, the underlying molecular mechanisms remain unclear. In the present study, we explored the expression of the PI3K/AKT signaling pathway in PGCs, investigated its effects on PGC self-renewal and biological properties, and identified the underlying mechanisms. Our findings indicated that although supplementation with the PI3K/AKT activator IGF-1 failed to promote proliferation under the assessed culture conditions, the PI3K/AKT inhibitor LY294002 resulted in retarded cell proliferation and reduced expression of germ cell-related markers. We further demonstrated that inhibition of PI3K/AKT regulates the cell cycle and promotes apoptosis in PGCs by activating the expression of BAX and inhibiting that of Bcl-2. These findings indicated that the PI3K/AKT pathway is required for cell renewal, apoptosis, and maintenance of the reproductive potential in chicken PGCs. This study aimed to provide a theoretical basis for the optimization and improvement of a culture system for chicken PGCs and provide insights into the self-renewal of vertebrate PGCs as well as potential evolutionary changes in this unique cell population.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11379996 | PMC |
| http://dx.doi.org/10.1016/j.psj.2024.104140 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Influence and Optimization of Diverse Culture Systems on Chicken Embryonic Stem Cell Culture.
Genes (Basel)
October 2024
Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.
Article Synopsis
- * Researchers explored different culture conditions using species-specific ESC media and small molecule compounds to optimize the derivation and maintenance of chicken ESC-like cells.
- * The R2i+LIF system, enhanced with additional cytokines, proved effective in creating chicken ESC-like clones with pluripotency markers and the ability to differentiate into germ layers, though more work is needed for long-term culture solutions.
Labeling and sorting of avian primordial germ cells utilizing Lycopersicon Esculentum lectin.
Dev Growth Differ
December 2024
Graduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan.
Avian species are essential resources for human society, with their preservation and utilization heavily dependent on primordial germ cells (PGCs). However, efficient methods for isolating live PGCs from embryos remain elusive in avian species beyond chickens, and even in chickens, existing techniques have shown limited efficiency. In this study, we present a rapid, simple, and cost-effective method for labeling and sorting circulating-stage PGCs across various avian species, including Carinatae and Ratitae, using Lycopersicon Esculentum (Tomato) lectin (LEL).
View Article and Find Full Text PDFSuccessful formation of sperm cells from transplanted primordial germ cells in sterile interspecific avian recipients.
Sci Rep
October 2024
National Centre for Biodiversity and Gene Conservation, Institute for Farm Animal Gene Conservation, Gödöllő, Hungary.
Article Synopsis
- * Transplanting PGCs into sterile hybrid hosts can facilitate gamete production without interference from the host's existing germ cells, presenting a simpler method than other procedures like busulphan treatment.
- * The study found that chicken PGCs injected into hybrid embryos resulted in nearly complete colonization of the gonads and the eventual production of mature sperm, although this process was slower than in standard domestic fowls, possibly due to hormonal factors.
Single-cell RNA sequencing reveals surface markers of primordial germ cells in chicken and zebra finch.
Mol Genet Genomics
September 2024
Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Korea.
Primordial germ cells (PGCs) in avian species exhibit unique developmental features, including the ability to migrate through the bloodstream and colonize the gonads, allowing their isolation at various developmental stages. Several methods have been developed for the isolation of avian PGCs, including density gradient centrifugation, size-dependent separation, and magnetic-activated cell sorting (MACS) or fluorescence-activated cell sorting (FACS) using a stage-specific embryonic antigen-1 (SSEA-1) antibody. However, these methods present limitations in terms of efficiency and applicability across development stages.
View Article and Find Full Text PDFPGC-based cryobanking, regeneration through germline chimera mating, and CRISPR/Cas9-mediated TYRP1 modification in indigenous Chinese chickens.
Commun Biol
September 2024
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan, 650201, China.
Primordial germ cells (PGCs) are vital for producing sperm and eggs and are crucial for conserving chicken germplasm and creating genetically modified chickens. However, efforts to use PGCs for preserving native chicken germplasm and genetic modification via CRISPR/Cas9 are limited. Here we show that we established 289 PGC lines from eight Chinese chicken populations with an 81.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!