is one of the most important mastitis-causing pathogens. Although the pathogenesis and virulence factors required for the intramammary infection development are not yet well established, several putative virulence-associated genes have been described. This work aimed to investigate the presence of ten known and putative virulence-associated genes in isolated from subclinical or clinical mastitis and its closely related species in 135 dairy farms in the Czech Republic. The PCR analysis detected that all the examined isolates possessed at least four virulence genes and most isolates carried eight out of ten virulence genes. All isolates were positive for the , and genes. Among the most prevalent virulence-associated genes (98%) and (97%) were also found. The and genes were always present together in 94% of the isolates. The genes and were detected in 6% and 2%, respectively. In the isolates, 14 different virulence gene profiles were observed. The most frequent profile was with variable , observed in 86% of the tested isolates, occurring in 127 out of 135 farms. was identified very sporadically and, although it is closely related to , only a rare occurrence of the examined virulence-associated genes was found.
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http://dx.doi.org/10.17221/95/2021-VETMED | DOI Listing |
Vet Microbiol
January 2025
Department of Animal Science, ETSEA, Universitat de Lleida, Lleida 25198, Spain. Electronic address:
Streptococcus suis (S. suis) is a major pathogen for pigs, causing large economic losses to the swine industry. Moreover, this bacterium has a zoonotic potential, being capable of infecting humans in close contact with pigs or, less frequently, through contact with pork products.
View Article and Find Full Text PDFVaccines (Basel)
December 2024
European Union Reference Laboratory for African Swine Fever (EURL), Centro de Investigación en Sanidad Animal (CISA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Consejo Superior de Investigaciones Científicas (CSIC), Valdeolmos, 28130 Madrid, Spain.
African swine fever virus (ASFV) is a devastating disease affecting domestic and wild suids and causing significant economic losses in the global pig industry. Attenuated modified live virus (MLV) vaccines are the most promising approaches for vaccine development. This study aimed to evaluate the safety and efficacy of four recombinant ASFV genotype II strains, derived from the non-hemadsorbing (non-HAD) attenuated isolate Lv17/WB/Rie1, through the single or simultaneous deletion of virulence-associated genes.
View Article and Find Full Text PDFMicroorganisms
December 2024
Department of Pathology, Clinical and Toxicological Analysis, Health Sciences Center, State University of Londrina, Londrina 86057-970, Paraná, Brazil.
is a significant pathogen responsible for infections in both humans and livestock, possessing various virulence mechanisms and antimicrobial resistance that make it even more concerning. In this study, several internationally recognized clones of were identified, such as ST131, ST38, ST648, and ST354, from chicken meat, pork, and human infection samples. Notably, ST131, belonging to phylogroup B2, was the dominant sequence type (ST) in human samples, while ST38, belonging to phylogroup D, was the most prevalent in meat samples.
View Article and Find Full Text PDFMicroorganisms
December 2024
Department of Biochemistry, Faculty of Science, Kasetsart University, 50 Ngam Wong Wan, Chatuchak, Bangkok 10900, Thailand.
Vibriosis caused by is a major problem in aquatic animals, particularly brown marble groupers (). biotype I has recently been isolated and classified into subgroups SUKU_G1, SUKU_G2, and SUKU_G3 according to the different types of virulence genes. In a previous study, we have shown that biotype I strains were classified into three subgroups according to the different types of virulence genes, which exhibited different phenotypes in terms of growth rate and virulence.
View Article and Find Full Text PDFAntibiotics (Basel)
December 2024
HUN-REN Veterinary Medical Research Institute, 1143 Budapest, Hungary.
: The One Health approach is crucial for managing and controlling the spread of antimicrobial resistance. is a recently identified bacterial species that seems to be a component of the oral microbiota of dogs; however, its pathogenic nature is questionable. : In this study, the antibacterial susceptibility of isolates was determined using the disk diffusion and broth microdilution methods.
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