is one of the most important mastitis-causing pathogens. Although the pathogenesis and virulence factors required for the intramammary infection development are not yet well established, several putative virulence-associated genes have been described. This work aimed to investigate the presence of ten known and putative virulence-associated genes in  isolated from subclinical or clinical mastitis and its closely related species in 135 dairy farms in the Czech Republic. The PCR analysis detected that all the examined isolates possessed at least four virulence genes and most isolates carried eight out of ten virulence genes. All isolates were positive for the , and genes. Among the most prevalent virulence-associated genes (98%) and (97%) were also found. The and genes were always present together in 94% of the isolates. The genes and were detected in 6% and 2%, respectively. In the  isolates, 14 different virulence gene profiles were observed. The most frequent profile was with variable , observed in 86% of the tested isolates, occurring in 127 out of 135 farms. was identified very sporadically and, although it is closely related to , only a rare occurrence of the examined virulence-associated genes was found.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11334765PMC
http://dx.doi.org/10.17221/95/2021-VETMEDDOI Listing

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