Purpose: To determine and compare the cytotoxicity, odontoblast-like differentiation, shear bond strength (SBS) and Vickers microhardness of four commercial light-cured orthodontic adhesives.
Methods: The orthodontic resins selected were Transbond XT - GI, Transbond Plus Color Change - GII (both from 3M Unitek), Enlight - GIII and Blugloo - GIV (both from Ormco). Samples were prepared, and leached monomers were obtained. Cytotoxicity was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and deposited calcium was analyzed using Alizarin red staining (ARS). SBS and the adhesive remnant index (ARI) were determined using 120 human premolars. The Vickers microhardness test was performed on the resin discs.
Results: All adhesives showed moderate to severe cytotoxicity (21-37%) and promoted similar formation of calcium deposits. A SBS of 6-8 MPa was achieved only by Blugloo (7.1 ± 2.4 MPa), and Enlight showed the lowest Vickers hardness score (40 ± 2.5 HV). Transbond Plus Color Change (score 0 = 42.9%) and Blugloo (score 0 = 46.4%) showed better ARI scores than Transbond XT (score 0 = 7.1%) and Enlight (score 0 = 3.6%).
Conclusion: On the basis of the properties evaluated, Blugloo seems to be the best option.
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http://dx.doi.org/10.2334/josnusd.24-0061 | DOI Listing |
J Toxicol Sci
December 2024
Stomatological Hospital, School of Stomatology, Southern Medical University, Guangzhou, People's Republic of China.
Numerous studies have confirmed that the apoptosis induced by the methacrylate resin monomers triethyleneglycol-dimethacrylate (TEGDMA), 2-hydroxy ethyl methacrylate (HEMA), etc., in pulp cells and odontoblast-like cells is caused mainly by oxidative stress (OS). Reactive oxygen species (ROS), recognized as the most important risk factor for apoptosis in cells of the pulp-dentin complex, are produced mainly via the mitochondrial respiratory chain.
View Article and Find Full Text PDFClin Oral Investig
September 2024
Department of Restorative Dentistry, Piracicaba Dental School, University of Campinas, 901 Limeira Avenue, Areião, Piracicaba, SP, 13414-903, Brazil.
Objective: To evaluate the efficacy and cytotoxicity of experimental 6% and 35% hydrogen peroxide gels (HP6 or HP35) incorporated with titanium dioxide nanoparticles (NP) co-doped with nitrogen and fluorine and irradiated with a violet LED light (LT).
Methods: Bovine enamel-dentin disks adapted to artificial pulp chambers were randomly assigned to bleaching (n = 8/group): NC (negative control), NP, HP6, HP6 + LT, HP6 + NP, HP6 + NP + LT, HP35, HP35 + LT, HP35 + NP, HP35 + NP + LT, and commercial HP35 (COM). Color (ΔE) and whiteness index (ΔWI) changes were measured before and 14 days after bleaching.
J Oral Sci
October 2024
Laboratory of Interdisciplinary Research, Area of Nanostructures and Biomaterials, National School of Higher Studies Unit Leon, National Autonomous University of Mexico.
Purpose: To determine and compare the cytotoxicity, odontoblast-like differentiation, shear bond strength (SBS) and Vickers microhardness of four commercial light-cured orthodontic adhesives.
Methods: The orthodontic resins selected were Transbond XT - GI, Transbond Plus Color Change - GII (both from 3M Unitek), Enlight - GIII and Blugloo - GIV (both from Ormco). Samples were prepared, and leached monomers were obtained.
Dent Mater
August 2024
Department of Morphology, Orthodontics, and Pediatric Dentistry, São Paulo State University (UNESP), School of Dentistry, Araraquara, Brazil. Electronic address:
Objectives: To investigate the transdentinal effects of surface reaction-type pre-reacted glass-ionomer (S-PRG) fillers on odontoblast-like cells.
Methods: An eluate of S-PRG fillers was obtained by dissolving the particles in distilled water (1:1 m/v). Dentin discs with similar permeability were mounted into artificial pulp chambers and MDPC-23 cells were seeded on their pulpal surface.
Int Endod J
September 2024
Department of Pediatric Dentistry, School and Hospital of Stomatology, China Medical University, Shenyang, China.
Aim: To evaluate the role of biomimetic pulp scaffolds derived from the extracellular matrix derived of stem cells from human exfoliated deciduous teeth (SHED-ECM-PS) in promoting pulp-dentine complex regeneration.
Methodology: SHED-ECM-PS was prepared through cell aggregation and decellularization techniques. Histological and immunofluorescence analyses, scanning electron microscopy, and DNA quantification assays were used to characterize the SHED-ECM-PS.
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