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Coactivator-independent vitamin D receptor signaling causes severe rickets in mice, that is not prevented by a diet high in calcium, phosphate, and lactose. | LitMetric

AI Article Synopsis

  • The vitamin D receptor (VDR) is essential for regulating bone health, and its activation typically involves binding to vitamin D and the recruitment of coactivators for gene transcription.
  • This study used mice with a deletion of the VDR-AF2 domain to explore how VDR functions without coactivators, revealing that these mutant mice had significant bone issues compared to regular knockout mice.
  • Findings indicated that while a rescue diet could improve some bone problems in one group of mutant mice, coactivator-independent VDR functions likely play a more vital role in organs other than bones, affecting overall mineral homeostasis.

Article Abstract

The vitamin D receptor (VDR) plays a critical role in the regulation of mineral and bone homeostasis. Upon binding of 1α,25-dihydroxyvitamin D to the VDR, the activation function 2 (AF2) domain repositions and recruits coactivators for the assembly of the transcriptional machinery required for gene transcription. In contrast to coactivator-induced transcriptional activation, the functional effects of coactivator-independent VDR signaling remain unclear. In humans, mutations in the AF2 domain are associated with hereditary vitamin D-resistant rickets, a genetic disorder characterized by impaired bone mineralization and growth. In the present study, we used mice with a systemic or conditional deletion of the VDR-AF2 domain (Vdr) to study coactivator-independent VDR signaling. We confirm that ligand-induced transcriptional activation was disabled because the mutant VDR protein was unable to interact with coactivators. Systemic Vdr mice developed short, undermineralized bones with dysmorphic growth plates, a bone phenotype that was more pronounced than that of systemic Vdr knockout (Vdr) mice. Interestingly, a rescue diet that is high in calcium, phosphate, and lactose, normalized this phenotype in Vdr, but not in Vdr mice. However, osteoblast- and osteoclast-specific Vdr mice did not recapitulate this bone phenotype indicating coactivator-independent VDR effects are more important in other organs. In addition, RNA-sequencing analysis of duodenum and kidney revealed a decreased expression of VDR target genes in systemic Vdr mice, which was not observed in Vdr mice. These genes could provide new insights in the compensatory (re)absorption of minerals that are crucial for bone homeostasis. In summary, coactivator-independent VDR effects contribute to mineral and bone homeostasis.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11335873PMC
http://dx.doi.org/10.1038/s41413-024-00343-7DOI Listing

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