Fruit maturity at harvest is a major factor in determining its quality. In this study, external skin color of grape has been utilized to predict their chemical content and, in turn, the maturity of the fruit. Measurements of the chemical content such as Brix and acidity were made on ten bunches of "Shine Muscat" grapes at three different harvest periods (immature, mature, and overmature). Using a machine vision system, color and UV-excited fluorescence images of grape berries and bunches were taken during the respective harvest stages. Acquired images were processed using ImageJ to obtain RGB values. Rratio and a*/b* are strongly related to Brix and sugar-to-acid ratio of grape fruit, with regression coefficients of 0.5626 and 0.5180, repectively. It was found that a* color index was the best predictor of grape bunch maturity. Furthermore, discriminant analysis has shown that color images of grape berries perform better than 365 nm fluorescence images.
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http://dx.doi.org/10.1016/j.foodchem.2024.140894 | DOI Listing |
Nat Commun
December 2024
Research Center for Applied Sciences, Academia Sinica, Taipei, 11529, Taiwan.
Taking advantage of the good mechanical strength of expanded Drosophila brains and to tackle their relatively large size that can complicate imaging, we apply potassium (poly)acrylate-based hydrogels for expansion microscopy (ExM), resulting in a 40x plus increased resolution of transgenic fluorescent proteins preserved by glutaraldehyde fixation in the nervous system. Large-volume ExM is realized by using an axicon-based Bessel lightsheet microscope, featuring gentle multi-color fluorophore excitation and intrinsic optical sectioning capability, enabling visualization of Tm5a neurites and L3 lamina neurons with photoreceptors in the optic lobe. We also image nanometer-sized dopaminergic neurons across the same intact iteratively expanded Drosophila brain, enabling us to measure the 3D expansion ratio.
View Article and Find Full Text PDFNat Commun
December 2024
Department of Convergence IT Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea.
Mid-infrared photoacoustic microscopy can capture biochemical information without staining. However, the long mid-infrared optical wavelengths make the spatial resolution of photoacoustic microscopy significantly poorer than that of conventional confocal fluorescence microscopy. Here, we demonstrate an explainable deep learning-based unsupervised inter-domain transformation of low-resolution unlabeled mid-infrared photoacoustic microscopy images into confocal-like virtually fluorescence-stained high-resolution images.
View Article and Find Full Text PDFthe axillary reverse mapping (ARM) procedure aims to preserve the lymphatic drainage structures of the upper extremity during axillary surgery for breast cancer, thereby reducing the risk of lymphedema in the upper limb. Material and this prospective study included 57 patients with breast cancer who underwent SLNB and ARM. The sentinel lymph node (SLN) was identified using a radioactive tracer.
View Article and Find Full Text PDFFEBS Lett
December 2024
Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
Fluorescence resonance energy transfer (FRET)-based biosensors are powerful tools for studying second messengers with high temporal and spatial resolution. FRET is commonly detected by ratio imaging, but fluorescence lifetime imaging microscopy (FLIM), which measures the donor fluorophore's lifetime, offers a robust and more quantitative alternative. We have introduced and optimized four generations of FRET sensors for cAMP, based on the effector molecule Epac1, including variants for either ratio imaging or FLIM detection.
View Article and Find Full Text PDFFront Cell Infect Microbiol
December 2024
School of Biosciences, University of Kent, Canterbury, United Kingdom.
Introduction: Antimicrobial resistance is a growing health problem. Pseudomonas aeruginosa is a pathogen of major concern because of its multidrug resistance and global threat, especially in health-care settings. The pathogenesis and drug resistance of depends on its ability to form biofilms, making infections chronic and untreatable as the biofilm protects against antibiotics and host immunity.
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