AI Article Synopsis
- * A new ratiometric fluorescent probe has been developed for super-resolution imaging, allowing for detailed visualization of LDs and HClO in foam cells at the nanometer level.
- * This probe can specifically and sequentially indicate the presence of LDs and HClO, and it effectively detects elevated HClO in atherosclerotic plaques, providing a promising tool for early assessments of atherosclerosis.
Article Abstract
Atherosclerosis (AS) is the leading cause of cardiovascular disease. Foam cells, with elevated lipid droplets (LDs) and HClO levels, are the main components of the atherosclerotic plaques that are characteristic of AS. Super-resolution imaging can be used to visualize the distribution of LDs in foam cells at the nanometer level, facilitating the identification of LDs and HClO. In the present study, we report the development of a ratiometric fluorescent probe, , for super-resolution imaging of LDs and HClO. Super-resolution imaging with this probe revealed the precise structure of LDs at the suborganelle level. Moreover, the fluorescence behavior of on the surface of LDs verified its excellent performance in detecting HClO in the foam cells. can sequentially and specifically respond to LDs and HClO "turn-on" and ratiometric signal output, respectively, thus contributing to precise imaging of foam cells. Importantly, we demonstrate that can be used to report on upregulated HClO in atherosclerotic plaques in the aorta of AS mice, providing a suitable fluorescent tool for early atherosclerotic disease assessment.
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| http://dx.doi.org/10.1021/acs.analchem.4c02881 | DOI Listing |
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