AI Article Synopsis
- - Wnt signaling is crucial for various cellular processes, and Xenopus laevis oocytes serve as an effective model to study these mechanisms, particularly for receptors like Frizzled 7 found in the CNS.
- - Using two-electrode voltage clamp recordings, researchers discovered that Wnt-5a affects the oocyte's membrane currents, resulting in changes to calcium-dependent currents, membrane depolarization, and alterations in potential and outward potassium currents.
- - Wnt-5a treatment improved oocyte viability and enhanced germinal vesicle breakdown compared to the control, indicating that Wnt-5a influences oocyte structure and signaling, contributing to a deeper understanding of the Wnt pathway's cellular effects.
Article Abstract
Wnt signaling plays an essential role in cellular processes like development, maturation, and function maintenance. Xenopus laevis oocytes are a suitable model to study not only the development but also the function of different receptors expressed in their membranes, like those receptors expressed in the central nervous system (CNS) including Frizzled 7. Here, using frog oocytes and recordings of endogenous membrane currents in a two-electrode path configuration along with morphological observations, we evaluated the role of the non-canonical Wnt-5a ligand in oocytes. We found that acute application of Wnt-5a generated changes in endogenous calcium-dependent currents, entry oscillatory current, the membrane's outward current, and induced membrane depolarization. The incubation of oocytes with Wnt-5a caused a reduction of the membrane potential, potassium outward current, and protected the ATP current in the epithelium/theca removed (ETR) model. The oocytes exposed to Wnt-5a showed increased viability and an increase in the percentage of the germinal vesicle breakdown (GVBD), at a higher level than the control with progesterone. Altogether, our results suggest that Wnt-5a modulates different aspects of oocyte structure and generates calcium-dependent endogenous current alteration and GVDB process with a change in membrane potential at different concentrations and times of the exposition. These results help to understand the cellular effect of Wnt-5a and present the use of Xenopus oocytes to explore the mechanism that could impact the activation of Wnt signaling.
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| http://dx.doi.org/10.1016/j.bbrc.2024.150507 | DOI Listing |
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