The high number of the strains of Escherichia coli (E. coli) isolated from faeces, demonstrated that this bacteria takes the first place in diarrhoea. These isolations had the same frequency all the year long. In infants, the enteropathogenic E. coli (E. P. E. C.) (28.57%) are still frequent contrary to developed countries. The E. coli with hemagglutination phenomenon (colonisation factors) but without toxin detected were also responsible (mortality 6%). The E. coli without toxin and not belonging to E. P. E. C. group were also frequent (71.42%). In adults, only the enteroinvasive E. coli (E. I. E. C.) (16.6%) and the E. coli with hemagglutination but without toxin detected (16.6%) were observed. This situation is due to bad hygienic conditions among the people and the tropical climate.
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Antibiotics (Basel)
November 2024
Department of Livestock and Pasture Science, University of Fort Hare, Alice 5700, South Africa.
Frequent use of colistin (COL) and tetracyclines in the Nigerian poultry sector potentially triggers bacterial resistance against COL and tigecycline (TIG), which are last-line antibiotics used to treat multidrug-resistant infections. This study aimed to isolate COL- and TIG-resistant . from commercial day-old chicks distributed to poultry farmers in Nsukka Southeastern Nigeria, assess the production of extended-spectrum β-lactamase (ESBL) and carbapenemase by the isolates, and establish their pathogenic potentials.
View Article and Find Full Text PDFGenes (Basel)
September 2024
School of Animal Science and Food Engineering, Jinling Institute of Technology, Nanjing 210046, China.
Background: Type 1 pili, as an important virulence factor of , has certain homology between APEC and UPEC, but the homology degree is not clear enough.
Objectives: This study aims to compare the homology between them.
Methods: The recombinant bacteria were constructed by homologous recombination.
BMC Microbiol
October 2024
Department of Microbiology and Immunology, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.
Background: Development of multidrug resistance in Uropathogenic Escherichia coli (UPEC) makes treatment of Urinary Tract Infections (UTIs) a major challenge. This study was conducted to investigate the effect of trans-resveratrol (t-RSV) at a subinhibitory concentration (sub-MIC-t-RSV) on phenotypic and genotypic expression of virulence factors of clinical isolates of UPEC and develop a nanoformulation of t-RSV. Fifty-five clinical UPEC strains were investigated for the presence of virulence factors by phenotypic methods and PCR detection of virulence genes.
View Article and Find Full Text PDFAntibodies (Basel)
August 2024
Atta-Ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, H-12 Sector, Islamabad 44000, Pakistan.
Critical blood shortages plague healthcare systems, particularly in lower-income and middle-income countries. This affects patients requiring regular transfusions and creates challenges during emergencies where universal blood is vital. To address these shortages and support blood banks during emergencies, this study reports a method for increasing the compatibility of blood group A red blood cells (RBCs) by blocking surface antigen-A using anti-A single chain fragment variable (scFv).
View Article and Find Full Text PDFFish Shellfish Immunol
October 2024
Hainan Key Laboratory of Tropical Aquatic Germplasm, Sanya Oceanographic Institution, Ocean University of China, Sanya, 572024, China; MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao, 266003, China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, 511458, China. Electronic address:
C-type lectins (CTLs) are a kind of Ca-dependent immunoreactive factors, which participated in pathogens recognition and defense. The present study identified a new CTL from hard clam Meretrix meretrix (designated as MmCTL4). The full-length of MmCTL4 cDNA was 608 bp, encoding a presumed signal peptide of 19 bp and a carbohydrate recognition domain (CRD) of 131 bp.
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