Near-infrared excitation Raman spectroscopy of colored fabric contaminated with body fluids.

Sci Rep

Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, 77843, USA.

Published: August 2024

Confirmatory identification of dyes in the physical pieces of evidence, such as hair and fabric, is critically important in forensics. This information can be used to demonstrate the link between a person of interest and a crime scene. High performance liquid chromatography is broadly used for dye analysis. However, this technique is destructive and laborious. This problem can be overcome by near-Infrared excitation Raman spectroscopy (NIeRS), non-invasive and non-destructive technique that can be used to determine chemical structure of highly fluorescent dyes. Analyzed fabric materials often possess body fluid stains, which may obscure the accuracy of NIeRS-based identification of dyes. In this study, we investigate the extent to which fabric contamination with body fluids can alter the accuracy of NIeRS. Our results showed that NIeRS coupled with partial-least squared discriminant analysis (PLS-DA) enabled on average 97.6% accurate identification of dyes on fabric contaminated with dry blood, urine and semen. We also found that NIeRS could be used to identify blood, urine and semen on such fabric with 99.4% accuracy. Furthermore, NIeRS could be used to differentiate between wet and dry blood, as well as reveal the presence of blood on washed fabric. These results indicate that NIeRS coupled with PLS-DA could be used as a robust and reliable analytical approach in forensic analysis of fabric.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11330518PMC
http://dx.doi.org/10.1038/s41598-024-70016-2DOI Listing

Publication Analysis

Top Keywords

identification dyes
12
near-infrared excitation
8
excitation raman
8
raman spectroscopy
8
fabric
8
fabric contaminated
8
body fluids
8
accuracy niers
8
niers coupled
8
dry blood
8

Similar Publications

The cosmetics industry is one of the fastest-growing sectors worldwide. The dynamic evolution of this industry results in an increasingly diverse range of products containing various active ingredients. Ensuring the quality of these products is crucial for consumer safety, necessitating the use of advanced analytical methods and adherence to legal regulations.

View Article and Find Full Text PDF

Purpose: This study aimed to evaluate the efficacy of indocyanine green (ICG)-fluorescence imaging for the identification of hepatic boundaries during liver resection and its advantages in surgical outcomes over conventional methods.

Methods: This prospective, exploratory, single-arm clinical trial included 47 patients with liver tumors who underwent liver resection using ICG-fluorescence imaging (ICG-LR) between 2019 and 2020. The primary outcome measure was the successful identification of hepatic boundaries during liver resection, from the perspective of both the hepatic surface and intrahepatic boundary, using ICG-fluorescence imaging.

View Article and Find Full Text PDF

Fluorescently Tagged Poly(methyl methacrylate)s.

Molecules

December 2024

Dipartimento di Chimica e Biologia, and INSTM Research Unit, Università di Salerno, Via Giovanni Paolo II 132, I-84084 Fisciano, SA, Italy.

Plastic pollution is a global problem affecting the environment and, consequently, people's well-being. Careful and timely end-of-life plastic recycling is certainly a way, albeit a partial one, to remedy the problem. The immediate identification and selection of the different types of plastic materials in the recycling process certainly facilitate its recovery and reuse, allowing the damage caused by plastic emission into the environment to be limited.

View Article and Find Full Text PDF

Engineered Strategies for Lipid Droplets-Targeted AIEgens Based on Tetraphenylethene.

Molecules

December 2024

School of Science and Engineering, Shenzhen Institute of Aggregate Science and Technology, The Chinese University of Hong Kong, Shenzhen 518172, China.

Lipid droplets (LDs), once regarded as inert fat particles, have been ignored by scientific researchers for a long time. Now, studies have shown that LDs are dynamic organelles used to store neutral lipids in cells and maintain cell stability. The abnormality of intracellular LDs usually causes metabolic disorders in the body, such as obesity, atherosclerosis, diabetes, and cancer, so the LDs have attracted wide attention.

View Article and Find Full Text PDF

Surface-enhanced Raman spectroscopy (SERS) is widely recognized as a powerful analytical technique, offering molecular identification by amplifying characteristic vibrational signals, even at the single-molecule level. While SERS has been successfully applied for a wide range of targets including pesticides, dyes, bacteria, and pharmaceuticals, it has struggled with the detection of molecules with inherently low Raman scattering cross-sections. Urea, a key nitrogen-containing biomolecule and the diamide of carbonic acid, is a prime example of such a challenging target.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!