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http://dx.doi.org/10.1093/pubmed/fdae214 | DOI Listing |
Genome Res
January 2025
Center for Genomic Research (CRG), Universitat Pompeu Fabra (UPF), ICREA
Nanopore direct RNA sequencing (DRS) enables direct measurement of RNA molecules, including their native RNA modifications, without prior conversion to cDNA. However, commercial methods for molecular barcoding of multiple DRS samples are lacking, and community-driven efforts, such as DeePlexiCon, are not compatible with newer RNA chemistry flowcells and the latest-generation GPU cards. To overcome these limitations, we introduce SeqTagger, a rapid and robust method that can demultiplex direct RNA sequencing datasets with 99% precision and 95% recall.
View Article and Find Full Text PDFNutrients
January 2025
Instituto Agroalimentario de Aragón (IA2), 50013 Zaragoza, Spain.
Background/objectives: Food deserts are areas characterized by limited access to affordable and healthy food, often due to significant distances from supermarkets-exceeding 1.6 km in urban areas and 16 km in rural settings. These spatial limitations exacerbate health and socioeconomic disparities.
View Article and Find Full Text PDFFront Digit Health
January 2025
Khoury College of Computer Sciences and Bouvé College of Health Sciences, Northeastern University, Boston, MA, United States.
Smartphones and wearable sensors offer an unprecedented ability to collect peripheral psychophysiological signals across diverse timescales, settings, populations, and modalities. However, open-source software development has yet to keep pace with rapid advancements in hardware technology and availability, creating an analytical barrier that limits the scientific usefulness of acquired data. We propose a community-driven, open-source peripheral psychophysiological signal pre-processing and analysis software framework that could advance biobehavioral health by enabling more robust, transparent, and reproducible inferences involving autonomic nervous system data.
View Article and Find Full Text PDFFront Oral Health
January 2025
School of Dentistry and Oral Health, College of Medicine, Nursing and Health Sciences, Fiji National University, Suva, Fiji.
Traditional dental research paradigms often lack relevance in marginalized cultural contexts due to inherent biases and misalignment with local values. For Pacific Islanders, this issue is pronounced, as they face serious oral health challenges while remaining underrepresented in scientific discourse. In response, the authors developed the Pacific Islands Dental Research Framework (PIDRF), a culturally informed, community-driven model that directly addresses these limitations in conventional Western approaches.
View Article and Find Full Text PDFImplement Sci
January 2025
Research group: Implementation Research, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
Background: The COVID-19 pandemic has highlighted the need for more effective immunization programs, including in limited resource settings. This paper presents outcomes and lessons learnt from a COVID-19 vaccination campaign (VC), which used a tailored adaptive strategy to optimise vaccine uptake in the Boeny region of Madagascar.
Methods: Guided by the Dynamic Sustainability Framework (DSF), the VC implementation was regularly reviewed through multi-sectoral stakeholder feedback, key informant interviews, problem-solving meetings, and weekly monitoring of outcome indicators to identify and apply key adaptations.
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