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Individual variation in the emergence of anterior-to-posterior neural fates from human pluripotent stem cells. | LitMetric

AI Article Synopsis

Article Abstract

Variability between human pluripotent stem cell (hPSC) lines remains a challenge and opportunity in biomedicine. In this study, hPSC lines from multiple donors were differentiated toward neuroectoderm and mesendoderm lineages. We revealed dynamic transcriptomic patterns that delineate the emergence of these lineages, which were conserved across lines, along with individual line-specific transcriptional signatures that were invariant throughout differentiation. These transcriptomic signatures predicted an antagonism between SOX21-driven forebrain fates and retinoic acid-induced hindbrain fates. Replicate lines and paired adult tissue demonstrated the stability of these line-specific transcriptomic traits. We show that this transcriptomic variation in lineage bias had both genetic and epigenetic origins, aligned with the anterior-to-posterior structure of early mammalian development, and was present across a large collection of hPSC lines. These findings contribute to developing systematic analyses of PSCs to define the origin and consequences of variation in the early events orchestrating individual human development.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11411333PMC
http://dx.doi.org/10.1016/j.stemcr.2024.07.004DOI Listing

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The combination of aminophylline and salbutamol is frequently used in clinical practice in the treatment of obstructive lung diseases. While the side effects (including arrhythmias) of the individual bronchodilator drugs were well described previously, the side effects of combined treatment are almost unknown. We aimed to study the arrhythmogenic potential of combined aminophylline and salbutamol treatment in vitro.

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Guidelines for managing and using the digital phenotypes of pluripotent stem cell lines.

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October 2024

Fraunhofer Institute for Biomedical Engineering (IBMT), Joseph-von-Fraunhofer Weg 1, 66280 Sulzbach, Germany; Berlin Institute of Health Center for Regenerative Therapies at Charité, Berlin, Germany. Electronic address:

Each pluripotent stem cell line has a physical entity as well as a digital phenotype, but linking the two unambiguously is confounded by poor naming practices and assumed knowledge. Registration gives each line a unique and persistent identifier that links to phenotypic data generated over the lifetime of that line. Registration is a key recommendation of the 2023 ISSCR Standards for the use of human stem cells in research.

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