AI Article Synopsis
- * Traditional detection methods such as bacterial colony counting and PCR are not sensitive enough for early-stage detection of S. aureus, indicating a need for improved techniques.
- * A new detection method using a SERS-based nanoplatform was developed, integrating aptamer-conjugated magnetic nanoparticles for enrichment and vancomycin-modified gold nanoparticles for quantitative analysis, achieving a limit of detection of 3.27 CFU/mL.
Article Abstract
Staphylococcus aureus (S. aureus) is the most common pathogen in human purulent infections, which can cause local purulent infections, as well as pneumonia, pseudomembranous enteritis, pericarditis, and even systemic infections. The conventional methods including bacteria colony counting, polymerase chain reaction and enzyme-linked immunosorbent assay can't fully meet the requirement of highly sensitive detection of S. aureus due to their own disadvantages. Therefore, it's an urgent need to develop new platform to detect S. aureus in the early infection stage. In this study, a new surface-enhanced Raman scattering (SERS)-based nanoplatform based on dual-recognition of aptamer (Apt) and vancomycin (Van) was developed for the highly sensitive detection of S. aureus. The SERS nanoplatform consisted of two functional parts: aptamer-conjugated FeO magnetic nanoparticles (FeO-Apt MNPs) for bacteria enrichment and vancomycin modified-Au nanoparticles (Van-Au NPs) as the SERS probes for S. aureus quantitative detection. Upon the target bacteria enrichment, the SERS signals of the supernatant after magnetic separation could be obtained and analyzed under different concentrations of S. aureus. The limit of detection of the proposed assay was found to be 3.27 CFU/mL. We believe that the proposed SERS-based nanoplatform has great potential as a powerful tool in the early detection of specific bacteria.
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| http://dx.doi.org/10.1016/j.talanta.2024.126691 | DOI Listing |
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