Bioactive substance contents and therapeutic potential for skin inflammation of an herbal gel containing and .

Context: Craib. and L., of the Fabaceae, have been used as active components in Thai herbal formulas for the treatment of fever and skin diseases.

Objective: To evaluate the physicochemical and pharmacological properties of the developed herbal gel formulation containing the combined extract from stem wood and (RGF).

Materials And Methods: The potential of the herbal gel formulation containing RGF (8% w/w) as the active ingredient was studied by evaluating the anti-inflammatory, antioxidant, and anti- activities using quantitative reverse transcription-polymerase chain reaction assay, spectrophotometric method, and broth microdilution technique, respectively. The reference standards for the biological testing included Nω-nitro-L-arginine (L-NA), ascorbic acid, catechin, and penicillin G. The stability study of the RGF herbal gel was performed by a heating-cooling test (at 45 °C for 24 h and at 4 °C for 24 h/1 cycle; for 6 cycles), and the bioactive marker compounds in the herbal gel were investigated by the HPLC technique.

Results: RGF showed promising pharmacological effects, particularly on its anti-inflammatory property (IC 73.86 µg/mL), compared to L-NA (IC 47.10 µg/mL). The RGF-containing gel demonstrated anti-inflammatory (IC 3.59 mg/mL) and free radical scavenging effects (IC 0.05-4.39 mg/mL), whereas it had no anti- activity (MIC > 10 mg/mL). The active ingredient in the developed herbal gel significantly inhibited lipopolysaccharide-induced nitric oxide production by downregulating iNOS mRNA levels. The contents of the bioactive markers in the RGF gel (lupinifolin and glabridin) did not change significantly after stability testing.

Discussion And Conclusions: The RGF-containing gel has potential to be further developed as an herbal product for the treatment of skin inflammation.