A Gene Cassette in Contains Two Genes that Affect Melanized Microsclerotium Formation and Virulence.

Phytopathology

Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs, and College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China.

Published: December 2024

AI Article Synopsis

  • - The text discusses a soilborne fungus that causes Verticillium wilt, affecting many plant species and highlights that while some of its genomes have been sequenced, functional gene studies are still in early stages.
  • - Previous research showed that a specific gene cassette was significantly more active in a mutant strain compared to a wild-type strain, leading to further investigation into its function.
  • - Experiments revealed that disrupting certain genes within this cassette hindered the fungus's reproduction and its ability to infect cotton plants, suggesting these genes are crucial for the fungus's growth and pathogenicity.

Article Abstract

is a soilborne phytopathogenic fungus causing Verticillium wilt on hundreds of plant species. Several sequenced genomes of . are available, but functional characterization of most genes has just begun. Based on our previous comparison of the transcriptome from the wild-type and Δ strains, a significant upregulation of the gene cassette, , in the Δ strain was observed. In this study, the functional characterization of the gene cassette was performed. -mediated knockout of this gene cassette in significantly inhibited conidiation, melanized microsclerotium formation in the mutant strains, and their virulence toward cotton. Furthermore, deletion of individual genes in the gene cassette identified that the disruption of and delayed microsclerotium formation, inhibited conidiation, and reduced virulence toward cotton. Our data suggest that and in the gene cassette mainly act as positive regulators of development and virulence in .

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Source
http://dx.doi.org/10.1094/PHYTO-11-23-0426-RDOI Listing

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