The objective of this experiment was to evaluate the effects of orally administered NCIMB 41125 as a microbial supplement in steers abruptly transitioned from a receiving diet with 4% dietary starch (dry matter [DM] basis) to a growing diet with 38% dietary starch (DM basis). Steers ( = 192; initial shrunk body weight [SBW] = 309 ± 20.6 kg) were assigned to microbial supplement treatment in a randomized complete block design. Treatments were control (CON): no microbial supplement prior to diet transition, and (DFM): microbial supplement orally administered prior to diet transition (20 mL of microbial supplement [Lactipro NXT, Axiota Animal Health, Fort Collins, CO] containing 1 × 10 colony forming units NCIMB 41125). Steers were sourced from a previously conducted 49 d feedlot receiving period experiment and abruptly transitioned from a receiving diet including soybean hulls and wheatlage containing 4% dietary starch (DM basis) to a growing diet including high-moisture ear corn, dry-rolled corn, and wheatlage containing 38% dietary starch (DM basis). Diets were switched on an equal DM intake basis to achieve the abrupt change and steers were fed the 38% starch diet for 49 d until experiment completion. Prior to experiment initiation, steers ( = 72;  = 3/pen) were fitted with wireless rumination tags to track daily activity and rumination time. No differences ( ≥ 0.20) were observed between treatments for final SBW, average daily gain, DM intake, feed efficiency, calculated net energy (NE) for maintenance and gain, or observed-to-expected ratio of NE for maintenance and gain. Additionally, no treatment × day or treatment differences ( ≥ 0.12) were observed for activity or rumination measures. Minutes ruminating and active both differed ( < 0.01) for the main effect of day. Compared to non-supplemented steers, oral administration of NCIMB 41125 did not improve growth performance or efficiency of dietary NE utilization in steers transitioned from a receiving diet containing 4% starch (DM basis) to a growing diet containing 38% starch (DM basis).

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