AI Article Synopsis
- Most mammalian cells possess circadian clocks that regulate the timing of various biological processes, but how they adapt to changes in metabolism is not well understood.* -
- This study utilized single-cell analysis to explore the relationship between circadian rhythms and protein stability without altering genes, focusing on key proteins involved in the circadian clock.* -
- Findings revealed that the duration of circadian rhythms adjusts based on the degradation rates of repressor proteins, with stability influenced by the phase of the circadian cycle, challenging existing theories about these mechanisms.*
Article Abstract
Most mammalian cells have molecular circadian clocks that generate widespread rhythms in transcript and protein abundance. While circadian clocks are robust to fluctuations in the cellular environment, little is known about the mechanisms by which the circadian period compensates for fluctuating metabolic states. Here, we exploit the heterogeneity of single cells both in circadian period and a metabolic parameter-protein stability-to study their interdependence without the need for genetic manipulation. We generated cells expressing key circadian proteins (CRYPTOCHROME1/2 (CRY1/2) and PERIOD1/2 (PER1/2)) as endogenous fusions with fluorescent proteins and simultaneously monitored circadian rhythms and degradation in thousands of single cells. We found that the circadian period compensates for fluctuations in the turnover rates of circadian repressor proteins and uncovered possible mechanisms using a mathematical model. In addition, the stabilities of the repressor proteins are circadian phase dependent and correlate with the circadian period in a phase-dependent manner, in contrast to the prevailing model.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11348271 | PMC |
| http://dx.doi.org/10.1073/pnas.2404738121 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Development of circadian rhythms in mammalian systems.
Biochem J
December 2024
Department of Pharmacology, Physiology, and Neurobiology, University of Cincinnati College of Medicine, Cincinnati, OH, U.S.A.
In mammals, molecular mechanisms of circadian rhythms involve a time-delayed negative feedback loop generating autonomous oscillations of ∼24 h. Most cell types in mammals possess circadian rhythms regulating temporal organization of cellular and physiological processes. Intriguingly, pluripotent stem cells do not possess circadian rhythms and oscillations arise after a defined period of differentiation.
View Article and Find Full Text PDFZebrafish Dark-Dependent Behavior Requires Phototransduction by the Pineal Gland.
J Pineal Res
November 2024
School of Neurobiology, Biochemistry and Biophysics, The George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel Aviv, Israel.
Located dorsally underneath a thin translucent skull in many teleosts, the pineal gland is a photoreceptive organ known as a key element of the circadian clock system. Nevertheless, the presence of additional routes of photoreception presents a challenge in determining its specific roles in regulating photic-related behavior. Here, we show the importance of the pineal gland in mediating a prolonged motor response of zebrafish larvae to sudden darkness, both as a photodetector and as a circadian pacemaker.
View Article and Find Full Text PDFTemporal therapy utilizing exosomes derived from M2 macrophages demonstrates enhanced efficacy in alleviating neuropathic pain in diabetic rats.
Korean J Pain
December 2024
School of Anesthesia, Shanxi Medical University, Shanxi, China.
Background: Diabetic pain patients have increased pain at night. Exosomes can relieve neuropathic pain. This study aimed to investigate the efficacy of exosome administration at different time points in relieving diabetic neuropathic pain (DNP) in rats.
View Article and Find Full Text PDFAn Automated Low-Cost Solution for Creating a Multiple-Step Thermal Gradient to Record Daily Fish Thermoregulatory Behavior.
Zebrafish
December 2024
Department of Life Sciences and Biotechnology, Faculty of Biology, University of Ferrara, Ferrara, Italy.
Photoperiod and temperature are two of the most powerful environmental cues that entrain circadian clocks. Being ectothermic, fish must keep their body temperature within a physiological range to optimize biological processes mainly applying behavioral strategies. Here, we developed a low-cost, automated system that allows to create a horizontal multiple-step thermal gradient and video record fish behavior for long-term periods.
View Article and Find Full Text PDFPerineuronal Nets in Syrian Hamsters: Anatomical Localization, Sex Differences, Diurnal Variation, and Response to Social Stress.
Brain Behav
December 2024
Neuroscience Institute, Georgia State University, Atlanta, Georgia, USA.
Purpose: Perineuronal nets (PNNs) are extracellular matrix proteoglycans surrounding neurons and glia. It has been suggested that PNNs are involved in the pathophysiology of multiple CNS illnesses, including stress-related neuropsychiatric disorders like schizophrenia, major depressive disorder, and anxiety disorders.
Method: Before examining the putative role of PNNs in stress-related responses, we described for the first time the anatomical distribution in Syrian hamsters (Mesocricetus auratus), an excellent model organism for studying social stress and circadian rhythms.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!