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| http://dx.doi.org/10.1186/s13028-024-00758-6 | DOI Listing |
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Hepatic infection in a dog with cavitary lung disease.
Can Vet J
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Central Victoria Veterinary Hospital, VCA Canada, 760 Roderick Street, Victoria, British Columbia V8X 2R3 (Xie, Seguin, Brownlee, Boller); Department of Veterinary Clinical and Diagnostic Sciences, Faculty of Veterinary Medicine, University of Calgary, 3280 Hospital Drive NW, Calgary, Alberta T2N 4Z6 (Boller).
A 9-year-old neutered male cairn terrier dog was initially presented because of inappetence, increased respiratory effort, and occasional coughing. A cavitary lung mass was diagnosed using CT and removed with lung lobectomy. Histopathology of the mass revealed necrosuppurative inflammation with acid-fast rod bacteria in macrophages, with spp.
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Department of Infectious Diseases, School of Translational Medicine, Monash University, Melbourne, Victoria, Australia.
Unlabelled: Sequencing DNA directly from patient samples enables faster pathogen characterization compared to traditional culture-based approaches, but often yields insufficient sequence data for effective downstream analysis. CRISPR-Cas9 enrichment is designed to improve the yield of low abundance sequences but has not been thoroughly explored with Oxford Nanopore Technologies (ONT) for use in clinical bacterial epidemiology. We designed CRISPR-Cas9 guide RNAs to enrich the human pathogen , by targeting multi-locus sequence type (MLST) and transfer RNA (tRNA) genes, as well as common antimicrobial resistance (AMR) genes and the resistance-associated integron gene .
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Department of Cardiology, The First Affiliated Hospital of Bengbu Medical University, Bengbu, China.
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Department of Microbiology & Virology, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy.
is a Non-Tuberculous Mycobacterium (NTM) belonging to the group, often associated with veterinary diseases, such as bovine farcy. However, it can also cause human infections and appears to be involved in Catheter-Associated Infections in immunocompromised patients. Here, we report the first Italian isolation of a strain of from a 16-year-old oncological female patient being treated at Fondazione IRCCS Policlinico San Matteo Pavia (Italy).
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Department of Research and Development, Pathnostics, Irvine, CA 92618, USA.
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