Authentication of true (genuine) cow leathers is in high demand to promote merchandise and economic growth. The present study employs RT-PCR-based TaqMan assay to facilitate the identification. Species-specific primers and probes were designed utilizing the existing NCBI data on mitochondrial DNA (mtDNA) genes, particularly the cytochrome b region (Cyt b). Mitochondrial DNA extracted from leather samples of both Bos taurus and Bos indicus and analyzed following the appropriate procedures. The RT-PCR results showed the designed primers and probes are exceptionally precise for cow leather samples. The established detection limit for the assay is estimated as 0.1 ng of DNA. In summary, the amplifiable mtDNA extracted from finished leather enables the identification of authentic cow leathers using the RT-PCR TaqMan assay, representing a pioneering report in this field.
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http://dx.doi.org/10.1016/j.mito.2024.101944 | DOI Listing |
Environ Sci Pollut Res Int
December 2024
Sant'Anna School of Advanced Studies, Institute of Management, Piazza Martiri Della Libertà 33, 56127, Pisa, Italy.
Int J Biol Macromol
December 2024
Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, China; National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, China. Electronic address:
Int J Biol Macromol
January 2025
University of Jeddah, Applied College, Biology Department, Jeddah, Saudi Arabia.
Microbial proteases and keratinases find extensive application in both the detergent and leather industries, as well as in poultry waste management. In this study, a multifunctional strain MH1 exhibiting proteolytic and keratinolytic activities was newly isolated and identified as Bacillus zhangzhouensis. To improve its stability, the proteolytic extract was spray-dried and the stability was assessed during two years of storage.
View Article and Find Full Text PDFJ Proteome Res
December 2024
College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
The species identification of leather artifacts is of great significance for studying the use and spread of ancient leathers; however, the absence of effective detection methods remains an obstacle. Here, we performed a shotgun proteomic analysis to identify the protein composition of ancient leather artifacts. Based on the Swiss-Prot database, 154 proteins were identified.
View Article and Find Full Text PDFEnviron Sci Pollut Res Int
October 2024
Faculty of Light Industry, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, China.
In this paper, cattle hair waste (CHW) was collected and physically milled into different meshes of ultrafine cattle hair powder (UCHP). The reuse of CHW reaches the purpose of treating pollution with waste by using bio-base adsorbent. It was characterized by using scanning electron microscope-energy dispersive spectrometer (SEM-EDS), Fourier transform infrared (FT-IR) spectroscopy, X-ray photoelectron spectroscopy (XPS), thermogravimetric analysis (TGA), differential scanning calorimeter (DSC), and X-ray diffraction (XRD).
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