AI Article Synopsis
- The study focuses on developing a sensitive biosensor to quickly detect spores of Bacillus anthracis, a potential biological terrorism agent.
- The biosensor uses magnetic beads and screen-printed electrodes to measure current changes, allowing for detection of live spore concentrations from 200 to 20,000 spores/ml.
- Results indicate high sensitivity and selectivity, with no interference from similar bacteria, making it a promising tool for rapid anthrax detection.
Article Abstract
Introduction: Due to most likely use of Bacillus anthracis in biological terrorism agents, the rapid and sensitive detection of its spores is crucial in both taking prophylactic measures and proper treatment. This study aimed to develop an amperometric electrochemical immunosensor for the detection of B. anthracis spores.
Methods: A new amperometric biosensor was designed using a combination of magnetic beads and multiplex screen-printed electrodes. This method measures changes in current intensity resulting from oxidation and reduction in the working electrode directly to spore concentrations.
Results: A standard curve was formed to test the number of live spores between 2 × 102-2 × 104 spores/ml concentrations. LOD and LOQ values were found to be 92 and 272 spores/ml, respectively. No cross-reactions were seen for Bacillus subtilis, Bacillus cereus and Bacillus thuringiencis spores.
Conclusions: It is shown that the designed Anthrax immunosensor has high sensitivity and selectivity with rapid detection results.
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| http://dx.doi.org/10.1016/j.diagmicrobio.2024.116473 | DOI Listing |
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