AI Article Synopsis
- The Ki-67 proliferative index (PI) is crucial for diagnosing nodal B-cell lymphoma (nBCL), but its evaluation in cytological samples lacks standardization.
- This study aimed to create a consistent method for accurately measuring Ki-67 PI in cytological slides to distinguish between less aggressive (indolent) and more aggressive forms of nBCL.
- The findings indicated a strong correlation between manual and flow-cytometry counting of lymphoma B cells and established specific cut-off values for Ki-67 PI using various counting methods, effectively differentiating between indolent and aggressive nBCLs.
Article Abstract
Background: The Ki-67 proliferative index (PI) is part of the diagnosis of nodal B-cell lymphoma (nBCL), but its determination in cytological samples is not standardized. We aimed to establish an approach for the accurate determination of the Ki-67 PI in cytological slides to differentiate between indolent and aggressive nBCLs.
Methods: Patients diagnosed with nBCL by fine-needle aspiration biopsy and subsequent excision biopsy were included. Cell suspensions were prepared from biopsy samples for CD3/Ki-67 double immunocytochemical staining and flow-cytometric verification of lymphoma B-cell counts. The Ki-67 PI was assessed by manual counting and eyeballing in cytology and eyeballing in histology. The cut-off values for the differentiation between aggressive and indolent lymphomas were determined for each method.
Results: A strong correlation between manual and flow-cytometric counting of lymphoma B cells was confirmed (interclass correlation coefficient (IC coef.) = 0.78). The correlation of the Ki-67 PI determined in cytological and histological slides was also strong (IC coef. > 0.80). Histologically, 55 cases were classified as indolent and 31 as aggressive nBCLs. KI-67 PI cut-off values of 28.5%, 27.5%, and 35.5% were established for manual counting and eyeballing in cytology and eyeballing in histology, respectively, with high sensitivity and specificity.
Conclusions: The Ki-67 PI, assessed by manual counting and eyeballing in cytological samples, accurately differentiates between indolent and aggressive nBCLs.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11311723 | PMC |
| http://dx.doi.org/10.3390/diagnostics14151584 | DOI Listing |
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