Association between Oral Microbiome and Gastroesophageal Reflux Severity.

Gastroesophageal reflux disease (GORD) is caused by gastric contents refluxing back into the oesophagus and oral cavity. It can lead to injuries to the mucosa in the form of erosion and ulcers. Our past research have shown acid reflux severity and disease progression is associated with alternations in the microbiota of the distal oesophagus. The aim of this study was to explore whether changes in the oral microbiota occurred in GORD patients and establish any associations with reflux severity. Fresh mouthwash samples were collected from 58 patients experiencing reflux symptoms referred for 24 h pH monitoring. The participants were categorised into three groups based on their DeMeester scores: Normal (<14.72), Mild (14.2-50), and Moderate/severe (>51). Microorganism identity and diversity were generated using hypervariable tag sequencing and analysing the V1-V3 region of the 16S rRNA gene. No differences in microbiota diversity were found in oral microbiota between groups using the Chiao1 diversity index and Shannon diversity index. Microbiota in the Mild group showed reductions in and , while and Clostridiales_1 were increased compared with the Normal group. In the Moderate/severe group, the abundance of was reduced compared with the Normal group, while , Rs_045, , , , and all had increased abundance. The abundance of was higher in the Mild group compared with Moderate/severe, while and abundances were lower. Our study shows the oral microbiome show significant differences between acid reflux severity groups, as categorised by DeMeester score.