In this issue of Structure, Kong et al. utilized cryoelectron tomography to closely examine Rubisco packaging within β-carboxysomes. They observed unique Rubisco packaging arrangements that may have important implications for carboxysome structural integrity.
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Rubisco packaging and stoichiometric composition of the native β-carboxysome in Synechococcus elongatus PCC7942.
Plant Physiol
December 2024
Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Crown Street, Liverpool L69 7ZB, United Kingdom.
Carboxysomes are anabolic bacterial microcompartments that play an essential role in CO2 fixation in cyanobacteria. This self-assembling proteinaceous organelle uses a polyhedral shell constructed by hundreds of shell protein paralogs to encapsulate the key CO2-fixing enzymes Rubisco and carbonic anhydrase. Deciphering the precise arrangement and structural organization of Rubisco enzymes within carboxysomes is crucial for understanding carboxysome formation and overall functionality.
View Article and Find Full Text PDFEngineering CO-fixing modules in E. coli via efficient assembly of cyanobacterial Rubisco and carboxysomes.
Plant Commun
December 2024
Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool L69 7ZB, United Kingdom; MOE Key Laboratory of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System & College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China. Electronic address:
Rubisco (ribulose 1,5-bisphosphate carboxylase/oxygenase) is the central enzyme for converting atmospheric CO into organic molecules, playing a crucial role in the global carbon cycle. In cyanobacteria and some chemoautotrophs, Rubisco complexes, along with carbonic anhydrase, are enclosed within specific proteinaceous microcompartments, known as carboxysomes. The polyhedral carboxysome shell ensures a dense packaging of Rubisco and creates a high-CO internal environment to facilitate the fixation of CO.
View Article and Find Full Text PDFRubisco packaging and stoichiometric composition of a native β-carboxysome.
bioRxiv
September 2024
Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Crown Street, Liverpool L69 7ZB, United Kingdom.
Carboxysomes are anabolic bacterial microcompartments that play an essential role in carbon fixation in cyanobacteria. This self-assembling proteinaceous organelle encapsulates the key CO-fixing enzymes, Rubisco and carbonic anhydrase, using a polyhedral shell constructed by hundreds of shell protein paralogs. Deciphering the precise arrangement and structural organization of Rubisco enzymes within carboxysomes is crucial for understanding the formation process and overall functionality of carboxysomes.
View Article and Find Full Text PDFUncovering the roles of the scaffolding protein CsoS2 in mediating the assembly and shape of the α-carboxysome shell.
mBio
October 2024
Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, United Kingdom.
Article Synopsis
- Carboxysomes are specialized protein organelles in cyanobacteria that enclose essential carbon-fixing enzymes like Rubisco and carbonic anhydrase within a shell structure.
- The scaffolding protein CsoS2 is crucial for their construction, with its N-terminal binding Rubisco and C-terminal promoting shell assembly, while the function of its middle region, CsoS2-M, was previously unclear.
- Recent findings show that CsoS2-M aids in the assembly and shaping of the α-carboxysome shell and is also involved in recruiting another variant of CsoS2, which is vital for encapsulating Rubisco, thus expanding our understanding of carboxysome biology and its potential in biotechnological applications.
Unraveling Rubisco packaging within β-carboxysomes.
Structure
August 2024
ARC Centre of Excellence in Synthetic Biology, Sydney, NSW, Australia; Discipline of Biological Sciences, School of Environmental and Life Sciences, University Drive, The University of Newcastle, Callaghan, NSW 2308, Australia.
In this issue of Structure, Kong et al. utilized cryoelectron tomography to closely examine Rubisco packaging within β-carboxysomes. They observed unique Rubisco packaging arrangements that may have important implications for carboxysome structural integrity.
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