AI Article Synopsis
- - A new strain of
- Pseudomonas juntendi
- (strain L4236), an opportunistic pathogen, was isolated from human feces and identified as multidrug resistant, though it remained sensitive to the antibiotic amikacin.
- - This strain was analyzed using various genomic and phenotypic methods, revealing a large genetic element known as a megaplasmid that carries resistance genes, including a novel variant.
- - The findings suggest that antimicrobial use may have contributed to the development of drug resistance in this strain, indicating a need for further research on its implications in clinical settings.
Article Abstract
Background: Pseudomonas juntendi is a newly identified opportunistic pathogen, of which we have limited understanding. P. juntendi strains are often multidrug resistant, which complicates clinical management of infection.
Methods: A strain of Pseudomonas juntendi (strain L4326) isolated from feces was characterized by MALDI-TOF-MS and Average Nucleotide Identity BLAST. This strain was further subject to whole-genome sequencing and Maximum Likelihood phylogenetic analysis. The strain was phenotypically characterized by antimicrobial susceptibility testing and conjugation assays.
Results: We have isolated the novel P. juntendi strain L4236, which was multidrug resistant, but retained sensitivity to amikacin. L4236 harbored a megaplasmid that encoded bla and a novel bla resistance gene variant. P. juntendi strain L4236 was phylogenetically related to P. juntendi strain SAMN30525517.
Conclusion: A rare P. juntendi strain was isolated from human feces in southern China with a megaplasmid coharboring bla and bla. Antimicrobial selection pressures may have driven acquisition of drug-resistance gene mutations and carriage of the megaplasmid.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11311922 | PMC |
| http://dx.doi.org/10.1186/s12864-024-10688-2 | DOI Listing |
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