Synergistic interactions between chemical inhibitors, whilst informative, can be difficult to interpret, as chemical inhibitors can often have multiple targets, many of which can be unknown. Here, using multiplexed transcriptional repression, we have validated that the simultaneous repression of glutamate racemase and alanine racemase has a synergistic interaction in . This confirms prior observations from chemical interaction studies and highlights the potential of targeting multiple enzymes involved in mycobacterial cell wall synthesis.
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http://dx.doi.org/10.1099/mic.0.001484 | DOI Listing |
J Biol Chem
November 2024
Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada. Electronic address:
Fusobacterium nucleatum is an oral commensal bacterium that can act as an opportunistic pathogen and is implicated in diseases such as periodontitis, adverse pregnancy outcomes, colorectal cancer, and Alzheimer's disease. F. nucleatum synthesizes lanthionine for its peptidoglycan, rather than meso-2,6-diaminopimelic acid (DAP) used by most Gram-negative bacteria.
View Article and Find Full Text PDFAppl Environ Microbiol
October 2024
Department of Biological Sciences, University of Illinois at Chicago, Chicago, Illinois, USA.
The symbiosis between and the Hawaiian bobtail squid, , is a tractable and well-studied model of bacteria-animal mutualism. Here, we developed a method to transiently colonize using D-alanine (D-ala) auxotrophy of the symbiont, controlling the persistence of viable infection by supplying or withholding D-ala. We generated alanine racemase () mutants of that lack avenues for mutational suppression of auxotrophy or reversion to prototrophy.
View Article and Find Full Text PDFMethods Mol Biol
August 2024
Department of Food Management, Miyagi University, Sendai, Japan.
The D-amino acids of D-alanine, D-glutamic acid, and D-aspartic acid increase tasting evaluation scores of Sake, a Japanese traditional alcohol beverage. Sake is brewed using seed mash for growth of brewing yeast without growth of contaminating microorganisms. Kimoto is brewed using lactic acid bacteria growth to decrease pH.
View Article and Find Full Text PDFMethods Mol Biol
August 2024
Department of Food Management, Miyagi University, Sendai, Japan.
A notable characteristic of amino acids is their optical isomerism, existing as L-form and D-form. Proteins are composed exclusively of L-form amino acids. However, recently, it is reported that D-alanine is evaluated particularly highly in terms of sensory evaluation.
View Article and Find Full Text PDFExtremophiles
August 2024
Graduate School of Pharmaceutical Sciences, Kitasato University, 5-9-1 Shirokane, Minato-Ku, Tokyo, 108-8641, Japan.
The peptidoglycan of the hyperthermophile Thermotoga maritima contains an unusual D-lysine in addition to the typical D-alanine and D-glutamate. Previously, we identified the D-lysine and D-glutamate biosynthetic pathways of T. maritima.
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