Prevalence and antimicrobial susceptibility pattern of complex isolates in Chongqing, Southwest China.

Heliyon

Laboratory of Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, National Center for Children's Health, 100045, China.

Published: July 2024

AI Article Synopsis

  • The study aimed to examine the prevalence and drug resistance of non-tuberculous mycobacteria (NTM), particularly Mycobacterium abscessus complex (MABC), in Chongqing, China, focusing on clarithromycin susceptibility.
  • Researchers collected 434 patient isolates and tested them for resistance to various antibiotics, finding that 22.6% were MABC, with significant resistance patterns observed involving specific mutations.
  • The findings suggest that certain genetic markers can help predict the susceptibility of MABC to macrolide antibiotics, which is crucial for treatment strategies.

Article Abstract

Objectives: To investigate the prevalence of complex (MABC), drug resistance characteristics, and the relationship between clarithromycin (CLA) susceptibility and MABC genotype in Chongqing, China.

Methods: A total of 434 NTM patient isolates were collected between October 2018 and October 2019. Isolates confirmed to be non-tuberculous mycobacteria (NTM) were tested for minimal inhibitory concentrations of antimicrobial agents. In addition, and gene sequences were used to analyze the acquired macrolide resistance and inducible macrolide resistance.

Results: Overall, 17 different NTM species were detected, of which (22.6 %, 91/403) was most prevalent. Amikacin, CLA, azithromycin and cefoxitin exhibited potent activities against MABC organisms, but no significant differences were observed in drug resistance rates between and ( > 0.05). On day 3 of culture, the acquired resistance rate against CLA was 7.4 % (9/121). Of 41 MABC isolates with inducible CLA resistant, 95.1 % (39/41) isolates belonged to the T28 sequevar, while the remaining 4.9 % (2/41) possessed the genotype. All C28 sequevar isolates were sensitive to CLA on day3 and day 14 of culture. Meanwhile, of the 5 T28 isolates with acquired resistance, all possessed 2058/2059 mutations, including 3 isolates with A2058C mutation and 2 isolates with A2059G mutation. While 2 of the 4  isolates with acquired resistance possessed the A2059G mutation, and one isolate possessed the A2058G mutation.

Conclusion: and gene could serve as useful markers for predicting macrolide susceptibility of MABC complex isolates.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11305262PMC
http://dx.doi.org/10.1016/j.heliyon.2024.e34546DOI Listing

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