AI Article Synopsis
- Gestational diabetes mellitus (GDM) poses risks to both mothers and infants, highlighting the need for early diagnostic biomarkers, with extrachromosomal circular DNA (eccDNA) being a promising candidate.
- In this study, plasma eccDNA profiles were analyzed in women who later developed GDM, identifying 2,217 differentially expressed eccDNAs, with PRDM16 showing significant potential for early diagnosis at 11-13 weeks of gestation.
- The findings suggest that eccDNA, particularly PRDM16, may serve as an effective biomarker for early screening of GDM, paving the way for improved maternal and infant health outcomes.
Article Abstract
Background: Gestational diabetes mellitus (GDM) significantly impacts maternal and infant health both immediately and over the long term, yet effective early diagnostic biomarkers are currently lacking. Thus, it is essential to identify early diagnostic biomarkers for GDM risk screening. Extrachromosomal circular DNA (eccDNA), being more stable than linear DNA and involved in disease pathologies, is a viable biomarker candidate for diverse conditions. In this study, eccDNA biomarkers identified for early diagnosis and assessment of GDM risk were explored.
Methods: Using Circle-seq, we identified plasma eccDNA profiles in five pregnant women who later developed GDM and five matched healthy controls at 11-13 weeks of gestation. These profiles were subsequently analyzed through bioinformatics and validated through outward PCR combined with Sanger sequencing. Furthermore, candidate eccDNA was validated by quantitative PCR (qPCR) in a larger cohort of 70 women who developed GDM and 70 normal glucose-tolerant (NGT) subjects. A ROC curve assessed the eccDNA's diagnostic potential for GDM.
Results: 2217 eccDNAs were differentially detected between future GDM patients and controls, with 1289 increased and 928 decreased in abundance. KEGG analysis linked eccDNA genes mainly to GDM-related pathways such as Rap1, MAPK, and PI3K-Akt, and Insulin resistance, among others. Validation confirmed a significant decrease in eccDNA PRDM16 in the plasma of 70 women who developed GDM compared to 70 NGT women, consistent with the eccDNA-seq results. PRDM16 showed significant diagnostic value in 11-13 weeks of gestation (AUC = 0.941, p < 0.001).
Conclusions: Our study first demonstrats that eccDNAs are aberrantly produced in women who develop GDM, including PRDM16, which can predict GDM at an early stage of pregnancy, indicating its potential as a biomarker.
Trial Registration: ChiCTR2300075971, http://www.chictr.org.cn . Registered 20 September 2023.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11304788 | PMC |
| http://dx.doi.org/10.1186/s12933-024-02381-1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Evaluation of polygenic risk scores for hormones and receptors levels in patients with vestibulodynia: a case-control study.
J Sex Med
January 2025
Clinical Obstetric and Gynecological V Buzzi, ASST-FBF-Sacco, Via Castelvetro 24-20124-University of the Study of Milan, Milan, Italy.
Background: Vulvodynia is a multifactorial disease affecting 7%-16% of reproductive-aged women in general population; however, little is still known about the genetics underlying this complex disease.
Aim: To compare polygenic risk scores for hormones and receptors levels in a case-control study to investigate their role in vulvodynia and their correlation with clinical phenotypes.
Methods: Our case-control study included patients with vestibulodynia (VBD) and healthy women.
Developing and testing the feasibility of a theory-based brief counseling intervention to promote physical activity in breast cancer survivors enrolled in the PAC-WOMAN trial.
Pilot Feasibility Stud
January 2025
CIDEFES Centro de Investigação em Educação Física, Desporto, Exercício e Saúde, Universidade Lusófona, Lisbon, Portugal.
Background: Despite the benefits of physical activity (PA), cancer survivors report engagement barriers, and existing interventions often lack comprehensive solutions. Theory-based interventions using evidence-based behavior change techniques (BCTs) have been shown to be effective in promoting PA for breast cancer survivors, although their feasibility and acceptability lack evidence. The PAC-WOMAN trial is a three-arm randomized controlled trial aimed at promoting short- and long-term PA and improving the quality of life of breast cancer survivors.
View Article and Find Full Text PDFWealth-related inequality in women healthcare-seeking behaviour for under-five children illness in Afghanistan: evidence from 2022 Afghanistan Multiple Indicator Cluster Survey.
BMC Health Serv Res
January 2025
Save the Children International, Qalai Fatullah, PD 10, Kabul, Afghanistan.
Background: This study examined the wealth-related inequality in women healthcare seeking behaviour for under-five children illness in Afghanistan and its determinants.
Methods: Data of 32409 mothers/caregivers of children under-five were extracted from Afghanistan Multiple Indicator Cluster Survey conducted in 2022. Wealth-related inequalities in women healthcare seeking behaviour for under-five children illness was investigated using Erreygers and Wagstaff concentration indices and curve.
Dropping out of a peripartum depression mHealth study: participants' motives and suggestions for improvement.
BMC Med Res Methodol
January 2025
Department of Women's and Children's Health - Obstetric & Reproductive Health Research, Uppsala University, Uppsala, 751 85, Sweden.
Background: Peripartum depression is a common but potentially debilitating pregnancy complication. Mobile applications can be used to collect data throughout the pregnancy and postpartum period to improve understanding of early risk indicators.
Aim: This study aimed to improve understanding of why women drop out of a peripartum depression mHealth study, and how we can improve the app design.
Molecule interacting with CasL-2 enhances tumor progression and alters radiosensitivity in cervical cancer.
J Transl Med
January 2025
Department of Radiation Oncology, The Second Affiliated Hospital of Dalian Medical University, No. 467 of Zhongshan Road, Shahekou District, Dalian, 116023, China.
Objective: Cervical cancer is a common malignancy among women, and radiotherapy remains a primary treatment modality across all disease stages. However, resistance to radiotherapy frequently results in treatment failure, highlighting the need to identify novel therapeutic targets to improve clinical outcomes.
Methods: The expression of molecule interacting with CasL-2 (MICAL2) was confirmed in cervical cancer tissues and cell lines through western blotting (WB) and immunohistochemistry (IHC).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!