Our study harnesses a CRISPR-based method to examine ecDNA biogenesis, uncovering efficient circularization between double-strand breaks. ecDNAs and their corresponding chromosomal scars can form via nonhomologous end joining or microhomology-mediated end joining, but the ecDNA and scar formation processes are distinct. Based on our findings, we establish a mechanistic model of excisional ecDNA formation.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11726015 | PMC |
| http://dx.doi.org/10.1158/2159-8290.CD-23-1117 | DOI Listing |
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