Background: Vahl. () is a medicinal plant containing anthraquinone compounds such as sennoside. is primarily valued for its laxative properties. In Persian medicine, this plant has been also used to treat various disorders such as diabetes and skin hyperpigmentation. Previous studies have shown that different species of , such as C. articulata, C. alata, , inhibit alpha-amylase and α-glucosidase enzymes. To the best of our knowledge, no previous evidence is available on tyrosinase and α-glucosidase inhibitory effects of the extract and different fractions of leaves.
Objectives: The purpose of this study was to investigate the inhibitory effect of the methanol-water extract and different fractions (hexane, chloroform, ethyl acetate, and remaining crude extract) of against tyrosinase and α-glucosidase and to investigate their total phenolic and sennoside B contents.
Results: Our findings depicted that the methanol-water extract and fractions had no significant anti-tyrosinase activity; however, some fractions were active toward α-glucosidase. The hexane fraction and the remaining crude extract demonstrated the highest inhibition on α-glucosidase compared to acarbose (positive control). In addition, the ethyl acetate fraction contains high phenolic and hydroxy anthraquinone derivatives based on the amount of sennoside B contents equivalent to 382.25 μg/mL of gallic acid and 1.525% of sennoside B, respectively. Moreover, no correlation was observed between the phenolic and sennoside contents of different fractions and their α-glucosidase inhibitory effect.
Conclusions: Considering the α-glucosidase inhibition results, the hexane fraction of can be a valuable fraction for in vitro and in vivo antidiabetic studies as well as further phytochemical studies. Further studies to identify the active substances and the exact mechanism of the bioactive ingredients on the inhibitory effects of α-glucosidase can provide promising results in the future.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11302428 | PMC |
| http://dx.doi.org/10.5812/ijpr-140914 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Vahl. Leaves: Determination of Total Phenolic and Sennoside Contents of Different Fractions in Comparison with Their α- Glucosidase and Tyrosinase Inhibitory Effects.
Iran J Pharm Res
March 2024
Department of Pharmacognosy, Faculty of Pharmacy and Persian Medicine and Pharmacy Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Background: Vahl. () is a medicinal plant containing anthraquinone compounds such as sennoside. is primarily valued for its laxative properties.
View Article and Find Full Text PDFUnveiling potential inhibitors targeting the nucleocapsid protein of SARS-CoV-2: Structural insights into their binding sites.
Int J Biol Macromol
July 2024
Protein Crystallography Section, Bhabha Atomic Research Centre, Mumbai, India; Homi Bhabha National Institute, Anushaktinagar, Mumbai, India. Electronic address:
The Nucleocapsid (N) protein of SARS-CoV-2 plays a crucial role in viral replication and pathogenesis, making it an attractive target for developing antiviral therapeutics. In this study, we used differential scanning fluorimetry to establish a high-throughput screening method for identifying high-affinity ligands of N-terminal domain of the N protein (N-NTD). We screened an FDA-approved drug library of 1813 compounds and identified 102 compounds interacting with N-NTD.
View Article and Find Full Text PDFMetabolomics analysis reveals the metabolite profiles of Rheum tanguticum grown under different altitudinal gradients.
BMC Plant Biol
March 2024
Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810008, China.
Background: Plant growth and quality are often affected by environmental factors, including geographical location, climate, and soil. In this study, we describe the effect of altitudinal differences on the growth and active ingredients in Rheum tanguticum Maxim. ex Balf.
View Article and Find Full Text PDFChronic Constipation in the United States: Results From a Population-Based Survey Assessing Healthcare Seeking and Use of Pharmacotherapy.
Am J Gastroenterol
June 2020
Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, California, USA.
Objectives: Chronic idiopathic constipation (CIC) is characterized by unsatisfactory defecation and difficult or infrequent stools. CIC affects 9%-20% of adults in the United States, and although prevalent, gaps in knowledge remain regarding CIC healthcare seeking and medication use in the community. We recruited a population-based sample to determine the prevalence and predictors of (i) individuals having discussed their constipation symptoms with a healthcare provider and (ii) the use of constipation therapies.
View Article and Find Full Text PDFIn vitro antioxidant and enzyme inhibitory properties of the n-butanol fraction of Senna podocarpa (Guill. and Perr.) leaf.
J Basic Clin Physiol Pharmacol
December 2019
Nutraceutical and Phytomedicine Research Laboratory, Biochemistry Programme, Department of Chemical Sciences, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria.
Background This study evaluates the antioxidant activity and enzyme inhibitory properties of the n-butanol fraction of Senna podocarpa leaves on α-amylase, α-glucosidase, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, arginase, phosphodiesterase 5 (PDE-5), and angiotensin converting enzyme (ACE). Methods The total phenol and flavonoids, iron (Fe) chelation, and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) free radical scavenging were used to determine the antioxidant activity, and the inhibitory activities of α-glucosidase, α-amylase, AChE, BChE, tyrosinase, arginase, PDE-5 and ACE were also assessed. Results The n-butanol fraction of S.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!