AI Article Synopsis
- Sun5, a testis-specific gene, is linked to acephalic spermatozoa syndrome (ASS) and plays a critical role in mRNA export in male germ cells.* -
- Knockout mice studies show that loss of Sun5 leads to accumulation of poly(A) RNA in nuclei, resulting in lower sperm counts and motility issues.* -
- Sun5 interacts with proteins like Nxf1 and Nup93, and disruption in this interaction impairs mRNA export, potentially explaining abnormal sperm development in affected mice.*
Article Abstract
, a testis-specific gene, is associated with acephalic spermatozoa syndrome (ASS). Here, we demonstrate that Sun5 is involved in mRNA export. In -knockout mice ( 5 ), poly(A) RNA accumulates in the nuclei of germ cells, leading to reduced sperm counts, decreased sperm motility and disrupted sperm head-to-tail junctions. Additionally, in the GC-2 germ cell line with RNA interference of , heterogeneous nuclear ribonucleoproteins (hnRNPs) and poly (A) RNA (mainly mRNA) are retained in the nucleus. Further mechanistic studies reveal that Sun5 interacts with Nxf1 (nuclear RNA export factor 1) and nucleoporin 93 (Nup93). Interference with Nup93 inhibits mRNA export. Treatment with leptomycin B to block the CRM1 pathway indicates that Sun5 regulates mRNA export through an Nxf1-dependent pathway. In mice, the binding of Nxf1 and Nup93 decreases due to loss of Sun5 function, and the process of submitting Nxf1-binding mRNPs to Nup93 is inhibited, resulting in abnormal spermatogenesis. Together, these data may elucidate a novel pathway for mRNA export in male germ cells.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11659784 | PMC |
| http://dx.doi.org/10.3724/abbs.2024134 | DOI Listing |
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