AI Article Synopsis
- PRMT6 plays a key role in plant immunity by targeting viral suppressor RNA silencing (VSR), which is crucial for virus infection.
- The study shows that manipulating PRMT6 expression in tomato plants affects disease symptoms during tomato bush stunt virus (TBSV) infection, highlighting its importance in viral resistance.
- PRMT6 inhibits the VSR function of TBSV by methylating specific arginine residues, which reduces the virus's ability to bind small RNAs and assemble effectively, thus enhancing overall plant defense.
Article Abstract
Viral suppressor RNA silencing (VSR) is essential for successful infection. Nucleotide-binding and leucine-rich repeat (NLR)-based and autophagy-mediated immune responses have been reported to target VSR as counter-defense strategies. Here, we report a protein arginine methyltransferase 6 (PRMT6)-mediated defense mechanism targeting VSR. The knockout and overexpression of PRMT6 in tomato plants lead to enhanced and reduced disease symptoms, respectively, during tomato bush stunt virus (TBSV) infection. PRMT6 interacts with and inhibits the VSR function of TBSV P19 by methylating its key arginine residues R43 and R115, thereby reducing its dimerization and small RNA-binding activities. Analysis of the natural tomato population reveals that two major alleles associated with high and low levels of PRMT6 expression are significantly associated with high and low levels of viral resistance, respectively. Our study establishes PRMT6-mediated arginine methylation of VSR as a mechanism of plant immunity against viruses.
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| http://dx.doi.org/10.1016/j.chom.2024.07.014 | DOI Listing |
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