AI Article Synopsis
- Salmonella is a leading zoonotic pathogen responsible for many global bacterial poisoning cases, making its detection in food a crucial public health concern.
- * Loop-mediated isothermal amplification (LAMP) has been developed over the past 20 years as a promising method for rapidly detecting Salmonella, offering distinct advantages over traditional techniques like polymerase chain reaction (PCR).
- * The paper reviews LAMP's technical aspects, its research progress in Salmonella detection, and summarizes key target genes for various Salmonella classifications, while also discussing potential developments for improving its application in food safety.
Article Abstract
Salmonella, the prevailing zoonotic pathogen within the Enterobacteriaceae family, holds the foremost position in global bacterial poisoning incidents, thereby signifying its paramount importance in public health. Consequently, the imperative for expeditious and uncomplicated detection techniques for Salmonella in food is underscored. After more than two decades of development, loop-mediated isothermal amplification (LAMP) has emerged as a potent adjunct to the polymerase chain reaction, demonstrating significant advantages in the realm of isothermal amplification. Its growing prominence is evident in the increasing number of reports on its application in the rapid detection of Salmonella. This paper provides a systematic exposition of the technical principles and characteristics of LAMP, along with an overview of the research progress made in the rapid detection of Salmonella using LAMP and its derivatives. Additionally, the target genes reported in various levels, including Salmonella genus, species, serogroup, and serotype, are summarized, aiming to offer a valuable reference for the advancement of LAMP application in Salmonella detection. Finally, we look forward to the development direction of LAMP and expect more competitive methods to provide strong support for food safety applications.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11303641 | PMC |
| http://dx.doi.org/10.1186/s11671-024-04075-9 | DOI Listing |
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