AI Article Synopsis
- The study focuses on the issue of high alloimmunization rates in sickle cell disease (SCD) patients, emphasizing the importance of accurately determining blood group genotypes to enhance blood compatibility and reduce risks.
- 104 SCD and sickle thalassemia patients were analyzed using Tetra ARMS PCR for Rh genotyping, revealing a variety of genotypes while highlighting discrepancies between molecular and serological methods in 11 cases.
- The findings suggest that combining Tetra ARMS PCR with traditional serological techniques is a significant advancement in immunohematology, improving patient care during blood transfusions.
Article Abstract
Objectives: High rate of alloimmunization in sickle cell disease (SCD) patients poses a significant challenge in finding compatible blood unit. Accurate determination of the blood group genotype of them can help reduce the alloimmunization risk. Tetra ARMS PCR is a novel method that has been utilized recently to investigate SNPs in diseases in a fast and reliable way.
Methods: Our study included 104 SCD and sickle thalassemia (Sβ) patients referred to Baghaei-2-Hospital of Ahvaz in 2019 using a nonrandom sampling method. Blood samples were collected for serological and molecular tests. Rh genotyping was performed using Tetra ARMS PCR and compared with the serological results.
Results: Based on the Tetra ARMS PCR method, out of 104 patients, 7 (6.7%) were d/d, 40 (38.5%) were D/d, 57 (54.8%) were D/D, 25 (24%) were C/C, 59 (56.7%) were C/c, 20 (19.3%) were c/c, 4 (3.8%) were E/E, 25 (24%) were E/e, and patients 75 (72.2%) were e/e. There were discrepancies in the serological and molecular results for 11 patients.
Conclusion: Use of Tetra ARMS PCR in combination with serological methods for determining the Rh blood group system in donors and transfusion-dependent patients represents a remarkable transformation in the field of immunohematology.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/1744666X.2024.2388700 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Interactions of spontaneous abortion with gene and dietary carotenoids; a case-control study.
J Nutr Sci
December 2024
Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Article Synopsis
- Spontaneous abortion (SA) is a common complication in pregnancy influenced by genetic factors and maternal nutrition.
- A study analyzed 192 women with a history of SA versus 347 healthy women, examining the impact of dietary carotenoids and specific gene polymorphisms on the occurrence of SA.
- The findings revealed that higher intake of β-cryptoxanthin might protect against SA only in women with the TT genotype of the related gene, while no significant links were found for other carotenoids.
Association between , , , and gene polymorphisms and breast cancer risk.
Per Med
December 2024
Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Aim: In this study, we examined the polymorphisms of (rs13252298, rs1456315), (rs4848320) (rs7158663), (rs7853346) genes in BC patients and compared it with healthy individuals in an Iranian population.
Method: The assessment of genetic polymorphisms was conducted using PCR - RFLP and PCR-Tetra ARMS methods.
Results & Conclusion: The results showed that Codominant, Dominant and G allele of rs13252298 polymorphism and Dominant of rs1456315 polymorphism are correlated with increased risk of BC.
Comparative Effectiveness of Buprenorphine/Naloxone and Methadone on Methamphetamine/Amphetamine Use Among People with Opioid Use Disorder in Canada.
Subst Use Addctn J
November 2024
British Columbia Centre on Substance Use, Vancouver, BC, Canada.
Article Synopsis
- The study investigates the impact of opioid agonist therapy (OAT) on methamphetamine and amphetamine use among individuals with opioid use disorder (OUD) in Canada, comparing buprenorphine/naloxone and methadone.
- Conducted over 24 weeks, data from a pan-Canadian trial highlighted that both treatments showed no significant effect on reducing methamphetamine/amphetamine use, as assessed through drug testing and self-reporting.
- Despite methamphetamine/amphetamine use being prevalent among participants, results indicated that neither treatment method led to notable changes in their usage patterns throughout the study period.
LncRNA MEG3, GAS5, and HOTTIP Polymorphisms Association with Risk of Polycystic Ovary Syndrome: A Case-Control Study and Computational Analyses.
Biochem Genet
November 2024
Cellular and Molecular Research Center, Research Institute of Cellular and Molecular Sciences in Infectious Diseases, Zahedan University of Medical Sciences, Zahedan, Iran.
As a multifactorial and endocrine disease, polycystic ovary syndrome (PCOS) affects approximately 5-20% of women worldwide. Recently, long noncoding RNAs (lncRNAs) have emerged as potent predictors of a particular phenotype in PCOS. Our preliminary study examines the link between polymorphisms in lncRNAs MEG3, HOTTIP, and GAS5 and the risk of PCOS.
View Article and Find Full Text PDFAssociation of lncRNA ANRIL rs10757278 A>G Variant, Tumor Size, Grading, Tumor Site, and Tumor Stage in Oral Squamous Cell Carcinoma Patients.
Rep Biochem Mol Biol
April 2024
Department of Biology, Zarghan Branch, Islamic Azad University, Zarghan, Iran.
Background: Oral Squamous Cell Carcinoma (OSCC) is a pressing global health challenge. Long non-coding RNAs (lncRNAs) have emerged as pivotal regulators. Among these, the lncRNA ANRIL (antisense non-coding RNA in the INK4 locus) has a role in cancer progression.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!