The application of a novel biomimetic enzyme p-BEs cascade catalytic platform for the rapid detection of glucose.

Int J Biol Macromol

Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; Supervision, Inspection and Testing Center for Fishery Environment and Aquatic Products (Harbin), Ministry of Agriculture and Rural Affairs, Harbin 150070, China. Electronic address:

Published: October 2024

AI Article Synopsis

  • The study focuses on the importance of blood glucose levels in aquatic organisms as an indicator of their health and presents a new biomimetic enzyme designed for detecting glucose in serum.
  • A portable system was developed to measure glucose levels using three different methods: quantitative analysis through UV-Vis/MD, on-site analysis with a mobile phone RGB, and semi-quantitative analysis with a drip plate.
  • The new approach offers improved detection limits (0.03 μM) and faster detection times (12 minutes) compared to existing methods, providing a cost-effective and easy-to-use solution for glucose monitoring.

Article Abstract

The blood glucose concentration in aquatic organisms, a crucial indicator reflecting their health status, holds significant importance for detecting glucose levels in serum in terms of processing and quality monitoring. In this study, a novel POD biomimetic enzyme (p-BEs) with horseradish peroxidase catalytic properties was designed, optimized, and its mechanism was discussed in detail. Based on this, a portable system has been developed capable of determining glucose levels in three ways: quantitatively analyzed through UV-Vis/MD, quantitatively analyzed on-site using a mobile phone RGB, and semi-quantitatively analyzed through a drip plate. Meanwhile, compared with other catalytic methods for detecting glucose, we achieved a lower limit of detection (0.03 μM) and shorter detection time (12 min), with high catalytic activity. This study provides new insights into the design of efficient and reliable cascade catalytic systems responsive to glucose, offering a low-cost, simplicity of operation method for glucose detection.

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http://dx.doi.org/10.1016/j.ijbiomac.2024.134485DOI Listing

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