Isolation and antimicrobial activity of secondary metabolites of wall.

The genus is extensively utilised in traditional medicine in China and India. An underexplored species of Wall was identified in Sulawesi, Indonesia. Antimicrobial activity was assessed using microdilutions and streak plates against , , , and . Significant effectiveness was observed in the methanol extract, as indicated by the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values for three different extracts (methanol, ethyl acetate, and -hexane) of . The isolates obtained were structurally analysed using Ultraviolet (UV)-spectroscopy, Fourier-transform Infra Red-Spectroscopy (FT-IR), Mass Spectroscopy (MS), Nuclear Magnetic Resonance (NMR), and antimicrobial testing after undergoing fractionation and subfractionation. The isolate obtained was stigmasterol with moderate antimicrobial activity against and , with MIC equivalent to MBC of 500 µg/ml. The first report of stigmasterol from has potent antimicrobial properties, bolstering empirical data in this field.