-Alkyl-2'-deoxyguanosine (-alkyl-dG) is a major type of minor-groove DNA lesions arising from endogenous metabolic processes and exogenous exposure to environmental contaminants. The -alkyl-dG lesions, if left unrepaired, can block DNA replication and transcription and induce mutations in these processes. Nevertheless, the repair pathways for -alkyl-dG lesions remain incompletely elucidated. By utilizing a photo-cross-linking coupled with mass spectrometry-based quantitative proteomic analysis, we identified a series of candidate -alkyl-dG-binding proteins. We found that two of these proteins, i.e., high-mobility group protein B3 (HMGB3) and SUB1, could bind directly to -Bu-dG-containing duplex DNA in vitro and promote the repair of this lesion in cultured human cells. In addition, HMGB3 and SUB1 protected cells against benzo[]pyrene-7,8-diol-9,10-epoxide (BPDE). SUB1 exhibits preferential binding to both the cis and trans diastereomers of -BPDE-dG over unmodified dG. On the other hand, HMGB3 binds favorably to --BPDE-dG; the protein, however, does not distinguish --BPDE-dG from unmodified dG. Consistently, genetic ablation of conferred diminished repair of --BPDE-dG, but not its counterpart, whereas loss of SUB1 conferred attenuated repair of both diastereomers. Together, we identified proteins involved in the cellular sensing and repair of minor-groove -alkyl-dG lesions and documented a unique role of HMGB3 in the stereospecific recognition and repair of -BPDE-dG.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11412153 | PMC |
http://dx.doi.org/10.1021/jacs.4c06680 | DOI Listing |
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