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[Anti-diabetic active constituents of pomegranate peel-derived extracellular nanovesicles]. | LitMetric

AI Article Synopsis

  • Pomegranate peel-derived extracellular nanovesicles (PPENs) were successfully isolated using advanced centrifugation techniques and characterized for their structure and morphology.
  • In vitro tests demonstrated that PPENs effectively inhibit α-glucosidase and improve insulin resistance in HepG2 cells, showing better results than the standard drug acarbose.
  • Comprehensive analyses revealed that PPENs are rich in lipids and proteins, with microRNA sequences identified, suggesting these components play crucial roles in their anti-diabetic effects through various biological pathways.

Article Abstract

Pomegranate peel-derived extracellular nanovesicles(PPENs) were isolated and purified by ultra-high speed centrifugation and sucrose density gradient centrifugation. Their morphology and structure were characterized. In vitro α-glucosidase inhibition assay and model test of insulin resistance(IR) in HepG2 cells showed that PPENs had good anti-diabetic activity. The IC_(50) value of α-glucosidase inhibition was(35.3±1.1) μg·mL~(-1), significantly better than the positive drug acarbose. At a concentration of 100 μg·mL~(-1), PPENs could increase the glucose absorption of IR cells significantly. Lipidome, proteome, and metabolite analysis of PPENs were performed using chromatography-mass spectrometry. MicroRNA(miRNA) sequences were identified, and target genes of miRNA were predicted. The analysis results indicated that PPENs contained abundant lipids and transport proteins, providing a material basis for the transportation and distribution of PPENs in tissue. Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis suggested that lipids and miRNAs may be the key components of PPENs to exert anti-diabetic activity.

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http://dx.doi.org/10.19540/j.cnki.cjcmm.20240405.201DOI Listing

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