Using a chemiluminescence reaction between luminol and HO in basic solution, an ultrasensitive electrochemiluminescence (ECL) aptasensor was developed for the determination of tobramycin (TOB), as an aminoglycoside antibiotic. TiC/Ni/Sm-LDH-based nanocomposite effectively catalyzes the oxidation of luminol and decomposition of HO, leading to the formation of different reactive oxygen species (ROSs), thus amplifying the ECL signal intensity of luminol, which can be used for the determination of TOB concentration. To evaluate the performance of the electrochemiluminescence aptasensor and synthesized nanocomposite, different methods such as cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) analyses were performed. The considerable specific area, large number of active sites, and enhanced electron transfer reaction on this nanocomposite led to the development of an ECL aptasensor with high sensitivity and electrocatalytic activity. After optimizing the preparation method and analysis conditions, the aptasensor revealed a wide linear response ranging from 1.0 pM to 1.0 μM with a detection limit of 18 pM, displaying outstanding accuracy, specificity, and response stability. The developed ECL sensor was found to be applicable to the determination of TOB in human serum samples and is anticipated to possess excellent clinical potentials for detecting other antibiotics, as well.
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http://dx.doi.org/10.1007/s00604-024-06536-5 | DOI Listing |
Mikrochim Acta
November 2024
Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, School of Petrochemical Engineering, Changzhou University, Changzhou, 213164, China.
An efficient "on-off-on" electrochemiluminescence (ECL) aptasensor utilizing dual-mechanism quenching was constructed for detecting furanyl fentanyl (FuF). The first signal "on" state was achieved by novel dual-ligand zinc metal-organic frameworks (Zn-MOFs), which were synthesized by self-assembly reaction using zinc atom clusters as metal nodes, achieving strong and stable ECL emission. The "off" state was realized by the energy and electron quenching effect of copper-doped WO.
View Article and Find Full Text PDFFood Chem
February 2025
Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Province Key Laboratory of Biomedical Materials and Chemical Measurement, Anhui Laboratory of Molecule-Based Materials, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China. Electronic address:
Exploring novel and sensitive analysis methods for monitoring lincomycin (Lin) residues is of great significance since overuse of it would cause a serious threat to public health. Herein, a Zr-porphyrin metal-organic frameworks (Zr-TCPP) with covalently modified polyamidoamine (PAMAM) dendrimers was synthesized as a novel intramolecular self-enhanced ECL reagent, which exhibited greatly improved ECL response due to the promotion of SO generation and the shortening of the electron transfer distance. Graphene oxide modified with gold nanoparticles (Au@GO) was synthesized as the quencher for the ECL sensor construction based on the quenching strategy.
View Article and Find Full Text PDFACS Appl Mater Interfaces
November 2024
Department of Pharmaceutics, School of Pharmacy, Nanjing Medical University, Nanjing 211166, China.
Anal Chem
November 2024
Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
ACS Sens
November 2024
Xi'an Key Laboratory of Functional Supramolecular Structure and Materials, Key Laboratory of Synthetic and Natural Functional Molecule of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi 710127, P. R. China.
An ultralow cathodic potential electrochemiluminescence (ECL) aptasensor was designed, employing DNA nanoribbon template self-assembly copper nanoclusters (DNR-CuNCs) as a novel coreaction accelerator within the luminol-HO system for the sensitive detection of kanamycin (KANA). Mechanistic investigations revealed that the DNR-CuNCs preferred to generate highly active hydroxyl radicals by facilitating the reduction of the coreactant HO under neutral pH conditions, consequently enhancing cathodic luminescence. By the strong π-π stacking effect of KANA aptamer and graphene as a signal modulation switch, DNR-CuNCs were displaced from the electrode surface due to the affinity of KANA and its aptamer, resulting in the inhibition of the luminol-HO system and a decrease in the ECL signal.
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