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Best practices for user consultation in flow cytometry shared resource laboratories. | LitMetric

AI Article Synopsis

  • The article outlines best practices in user consultation for flow cytometry, based on a survey and discussions from the ISAC community at the CYTO 2022 workshop.
  • It emphasizes the importance of starting consultations early in projects, especially for trainees, to enhance planning and data interpretation, reducing bias and saving time.
  • Additionally, it provides guidelines for effective communication and collaboration between shared research laboratories (SRLs) and users, helping researchers achieve reproducible and scientifically rigorous experiments.

Article Abstract

This "Best Practices in User Consultation" article is the result of a 2022 International Society for the Advancement of Cytometry (ISAC) membership survey that collected valuable insights from the shared research laboratory (SRL) community and of a group discussion at the CYTO 2022 workshop of the same name. One key takeaway is the importance of initiating a consultation at the outset of a flow cytometry project, particularly for trainees. This approach enables the improvement and standardization of every step, from planning experiments to interpreting data. This proactive approach effectively mitigates experimental bias and avoids superfluous trial and error, thereby conserving valuable time and resources. In addition to guidelines, the optimal approaches for user consultation specify communication channels, methods, and critical information, thereby establishing a structure for productive correspondence between SRL and users. This framework functions as an exemplar for establishing robust and autonomous collaborative relationships. User consultation adds value by providing researchers with the necessary information to conduct reproducible flow cytometry experiments that adhere to scientific rigor. By following the steps, instructions, and strategies outlined in these best practices, an SRL can readily tailor them to its own setting, establishing a personalized workflow and formalizing user consultation services. This article provides a pragmatic guide for improving the caliber and efficacy of flow cytometry research and aggregates the flow cytometry SRL community's collective knowledge regarding user consultation.

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Source
http://dx.doi.org/10.1002/cyto.a.24891DOI Listing

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