AI Article Synopsis
- Researchers have engineered an E. coli strain with five enzymes to transform vanillin from lignin into high-value cis,cis-muconic acid (ccMA) using green biotechnology.
- They optimized the production process in a bioreactor, achieving a significant yield of 5.2 g/L of ccMA in 48 hours by pulse-feeding vanillin.
- The ccMA produced is a key ingredient for creating a fully biodegradable polymer with rubber-like characteristics, emphasizing a sustainable approach to generating bio-based materials from renewable resources.
Article Abstract
Production of the high industrial value cis,cis-muconic acid (ccMA) from renewable biomasses is of main interest especially when biological (green) processes are used. We recently generated a E. coli strain expressing five recombinant enzymes to convert vanillin (VA, from lignin) into ccMA. Here, we optimized a growing cell approach in bioreactor for the ccMA production. The medium composition, fermentation conditions, and VA addition were tuned: pulse-feeding VA at 1 mmol/h allowed to reach 5.2 g/L of ccMA in 48 h (0.86 g ccMA/g VA), with a productivity 4-fold higher compared to the resting cells approach, thus resulting in significantly lower E-factor and Process Mass Intensity green metric parameters. The recovered ccMA has been used as building block to produce a fully bioderived polymer with rubber-like properties. The sustainable optimized bioprocess can be considered an integrated approach to develop a platform for bio-based polymers production from renewable feedstocks.
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| http://dx.doi.org/10.1016/j.biortech.2024.131190 | DOI Listing |
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