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Division of Biological Sciences, Section of Ecology, Behavior, and Evolution, University of California San Diego, La Jolla, CA 92093, USA. Electronic address:
Published: September 2024
AI Article Synopsis
The stop signal in honey bees is triggered by negative experiences at food sources and prevents them from performing waggle dances.
The protocol outlines how to measure the impact of danger signals on dopamine levels in honey bees through methods like observing colonies and simulating hornet attacks.
It includes detailed instructions for recording bee behaviors and measuring brain dopamine levels under varying conditions, with additional information available in the cited study by Dong et al.
Article Abstract
The stop signal is produced in response to negative experiences at the food source and inhibits honey bee (Apis mellifera) waggle dancing. Here, we present a protocol for measuring the effects of an inhibitory signal associated with danger on honey bee dopamine levels. We describe steps for observing honey bee colonies, training them with artificial nectar, and simulating hornet attacks. We then detail procedures for recording waggle dancing and stop signals and measuring brain dopamine levels during different treatments. For complete details on the use and execution of this protocol, please refer to Dong et al..
